GTX12865

antibody from GeneTex

Targeting: RPS6 S6

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX12865

Provider

GeneTex

Proper citation

GeneTex Cat#GTX12865, RRID:AB_367026

Product name

RPS6 (phospho Ser244/247) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 [pSpS244/247] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab¡¦)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to ribosomal protein S6 [pSpS244/247] blocks the signal, verifying the specificity of the antibody.