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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM32492SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti RPS6 pSer235
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide containing the sequence â¦RRL[pS]SL⦠in which pS corresponds to Phospho-Serine at residue 235 of Human ribosomal protein S6.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- MC27
- Vial size
- 0.1 ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western Blot Analysis: 3T3 cell lysate from serum starved cells (lane 1), and serum-stimulated cells (lane 2) were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with anti-phospho-Ribosomal Protein S6, MC27 (1:1,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and achemiluminescence detection system. Arrow indicates phosphorylated ribosomal protein S6 (30 kDa).
