Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
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Validation data
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- Product number
- 44-921G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-S6 (Ser236) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition. Lysates prepared from HEK293 cells left untreated (1) or treated with EGF (2-6) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 (pS236) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the phosphopeptide corresponding to ribosomal protein S6 (pSpS244/247) (3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ribosomal protein S6 (pS236) blocks the signal, verifying the specificity of the antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition. Lysates prepared from HEK293 cells left untreated (1) or treated with EGF (2-6) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 (pS236) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the phosphopeptide corresponding to ribosomal protein S6 (pSpS244/247) (3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ribosomal protein S6 (pS236) blocks the signal, verifying the specificity of the antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition. Lysates prepared from HEK293 cells left untreated (1) or treated with EGF (2-6) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 (pS236) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the phosphopeptide corresponding to ribosomal protein S6 (pSpS244/247) (3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ribosomal protein S6 (pS236) blocks the signal, verifying the specificity of the antibody.