44-921G

antibody from Invitrogen Antibodies

Targeting: RPS6 S6

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

44-921G

Provider

Invitrogen Antibodies

Product name

Phospho-S6 (Ser236) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Peptide Competition. Lysates prepared from HEK293 cells left untreated (1) or treated with EGF (2-6) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 (pS236) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the phosphopeptide corresponding to ribosomal protein S6 (pSpS244/247) (3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ribosomal protein S6 (pS236) blocks the signal, verifying the specificity of the antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Peptide Competition. Lysates prepared from HEK293 cells left untreated (1) or treated with EGF (2-6) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 (pS236) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the phosphopeptide corresponding to ribosomal protein S6 (pSpS244/247) (3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ribosomal protein S6 (pS236) blocks the signal, verifying the specificity of the antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Peptide Competition. Lysates prepared from HEK293 cells left untreated (1) or treated with EGF (2-6) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 (pS236) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the phosphopeptide corresponding to ribosomal protein S6 (pSpS244/247) (3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ribosomal protein S6 (pS236) blocks the signal, verifying the specificity of the antibody.