PA5-36759

antibody from Invitrogen Antibodies

Targeting: RPS6 S6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36759

Provider

Invitrogen Antibodies

Product name

Phospho-S6 (Ser240) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 29 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-S6 (Ser240) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with insulin (20 ng/mL) for 15 minutes whole cell lysate (40 µg), Lane 3: HEK293T treated with insulin (20 ng/mL) for 30 minutes whole cell lysate (40 µg). Samples were incubated with Phospho-S6 (Ser240) polyclonal antibody (Product # PA5-36759) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-S6 (Ser240) in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: HeLa treated with serum starved (24h) whole cell lysate (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: PC12 treated with serum starved (24h) whole cell lysate (40 µg). Samples were incubated with Phospho-S6 (Ser240) polyclonal antibody (Product # PA5-36759) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-S6 (Ser240) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-S6 (Ser240) polyclonal antibody (Product # PA5-36759) at a dilution of 1:100.