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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- F52532 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RPS6 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific RPS6 antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry/Immunofluorescence/ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 981CT2.1.1
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the RPS6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 1) 293, 2) HeLa, 3) mouse NIH3T3, 4) rat PC-12 and 5) rat brain tissue using RPS6 antibody at 1:2000. Predicted molecular weight ~29 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of HeLa cells stained with RPS6 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain section using RPS6 antibody at 1:25 dilution.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney section using RPS6 antibody at 1:25 dilution.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse kidney section using RPS6 antibody at 1:25 dilution.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using RPS6 antibody (green) and an isotype control of mouse IgG1 (blue); Ab was diluted at 1:25 dilution.
