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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX132281 - Provider product page
- Provider
- GeneTex
- Product name
- RPS6 (phospho Ser235) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) 293T whole cell extracts (starvation for 16 hrs; 40 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser235/236) antibody (GTX132281) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser235) antibody (GTX132281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RPS6 (phospho Ser235) antibody detects RPS6 (phospho Ser235) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPS6 (phospho Ser235) antibody (GTX132281) diluted at 1:500.
