GTX32351

antibody from GeneTex

Targeting: RPS6 S6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX32351

Provider

GeneTex

Product name

RPS6 (phospho Ser235) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Storage

Shipped at 4 ¢XC. Upon delivery aliquot and store at -20 ¢XC for one year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of RPS6 (pS235) expression in HeLa Calyculin A-treated (A); NIH3T3 (B) whole cell lysates.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of RPS6 (pS235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).