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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- ARG55752 - Provider product page
- Provider
- Arigo
- Product name
- anti-S6 Ribosomal Protein antibody
- Antibody type
- Monoclonal
- Antigen
- Human S6 Ribosomal recombinant protein
- Description
- Purification with Protein G.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 981CT2.1.1
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of 293, HeLa, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate (from left to right) stained with ARG55752 anti-S6 Ribosomal Protein antibody at 1:2000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG55752 anti-S6 Ribosomal Protein antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. brain section stained with ARG55752 anti-S6 Ribosomal Protein antibody at 1:25 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. kidney section stained with ARG55752 anti-S6 Ribosomal Protein antibody at 1:25 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded M. kidney section stained with ARG55752 anti-S6 Ribosomal Protein antibody at 1:25 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG55752 anti-S6 Ribosomal Protein antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor? 488 labelled secondary antibody.
