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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3819 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3819, RRID:AB_1580277
- Product name
- RPS6 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RPS6.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The organization of the intron-containing human S6 ribosomal protein (rpS6) gene and determination of its location at chromosome 9p21.
Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6.
The primary structure of the human ribosomal protein S6 derived from a cloned cDNA.
Antoine M, Fried M
Human molecular genetics 1992 Nov;1(8):565-70
Human molecular genetics 1992 Nov;1(8):565-70
Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6.
Lott JB, Mackie GA
Gene 1988 May 15;65(1):31-9
Gene 1988 May 15;65(1):31-9
The primary structure of the human ribosomal protein S6 derived from a cloned cDNA.
Heinze H, Arnold HH, Fischer D, Kruppa J
The Journal of biological chemistry 1988 Mar 25;263(9):4139-44
The Journal of biological chemistry 1988 Mar 25;263(9):4139-44
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of RPS6 polyclonal antibody (Cat # PAB3819) in HL-60 cell line lysates (35 ug/lane). RPS6 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of RPS6 (arrow) using rabbit RPS6 polyclonal antibody (Cat # PAB3819). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with RPS6 polyclonal antibody (Cat # PAB3819) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
