Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 46-9007-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse). Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens. Phosphorylation of ribosomal protein S6 by p70S6K and PKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry. Ribosomal protein S6 is dephosphorylated upon growth arrest.
- Antibody clone number
- cupk43k
- Concentration
- 5 µL/Test
Submitted references Novel GM-CSF signals via IFN-γR/IRF-1 and AKT/mTOR license monocytes for suppressor function.
Ribechini E, Hutchinson JA, Hergovits S, Heuer M, Lucas J, Schleicher U, Jordán Garrote AL, Potter SJ, Riquelme P, Brackmann H, Müller N, Raifer H, Berberich I, Huber M, Beilhack A, Lohoff M, Bogdan C, Eyrich M, Hermanns HM, Geissler EK, Lutz MB
Blood advances 2017 Jun 13;1(14):947-960
Blood advances 2017 Jun 13;1(14):947-960
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Intracellular staining of freshly-harvested (left), unstimulated (middle), or 30-minute LPS-stimulated (right) normal human peripheral blood cells with Anti-Human CD14 APC (Product # 17-0149-42) and Anti-Human/Mouse phospho-S6 Ribosomal (S235/S236) PerCP-eFluor® 710, using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total cells were used for analysis.