MA5-15123

antibody from Invitrogen Antibodies

Targeting: RPS6 S6

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15123

Provider

Invitrogen Antibodies

Product name

S6 Monoclonal Antibody (C.896.4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat, Drosophila

Host

Mouse

Isotype

IgG

Antibody clone number

C.896.4

Vial size

100 µL

Concentration

4 µg/mL

Storage

-20°C

References

Submitted references

Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy.

Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, Jablonka S, Sauer M, Moradi M, Sendtner M
Translational neurodegeneration 2022 Jun 2;11(1):31

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Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein.

Spena S, Cordiglieri C, Garagiola I, Peyvandi F
International journal of molecular sciences 2021 Feb 18;22(4)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of S6 Ribosomal Protein in HeLa cells using a S6 Ribosomal Protein monoclonal antibody (Product # MA5-15123) (red). Actin filaments have been labeled with FITC-conjugated phalloidin. DNA is labeled using a fluorescent blue dye.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of S6 Ribosomal Protein in NIH/3T3 cells using a S6 Ribosomal Protein monoclonal antibody (Product # MA5-15123) (blue) compared to a nonspecifc negative control antibody (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Ribosome responsiveness to the BDNF/TrkB signaling as well as ribosome/ER tethering are impaired in Smn-deficient axon terminals. a BDNF-stimulated Smn + / + ;SMN2tgtg and Smn -/- ;SMN2tgtg motoneurons expressing mCherry-KDEL were stained against RPL24, RPS6 and mCherry-KDEL. Growth cones were imaged by SIM. White boxes indicate enlarged ROIs within growth cones. In control but not Smn -/- ;SMN2tgtg motoneurons, BDNF induces formation of RPL24/RPS6 co-clusters that colocalize with the axonal ER at 10 s and 1-min poststimulation. b Smn -/- ;SMN2tgtg motoneurons do not exhibit increased number of RPL24/RPS6 co-clusters upon stimulation (n.s., P = 0.9999; n = 21-23 cells), in contrast to Smn + / + ;SMN2tgtg (* P = 0.0147 for no BDNF vs. 10-s BDNF; * P = 0.0210 for no BDNF vs. 1-min BDNF; n = 13-16 cells from 3 independent experiments). In control experiments, either RPL24 or RPS6 antibodies were omitted to exclude the crosstalk between channels. Colocalization analysis detected almost no co-clusters in the growth cones of control neurons (see also Additional file 1 : Fig. S3). c In Smn + / + ;SMN2tgtg motoneurons, the number of RPL24/RPS6 co-clusters that colocalize with ER increases significantly within 10 s and 1-min of stimulation (** P = 0.0089 for no BDNF vs. 10 s BDNF; ** P = 0.0092 for no BDNF vs. 1-min BDNF; n = 13-16 cells from 3 independent experiments). In Smn -/- ;SMN2tgtg motoneurons the number of RPL24/RPS6 co-clusters that associate with ER does not increase upon