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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-16398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-S6 (Ser240, Ser244) Monoclonal Antibody (SP45)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is prostate or lung squamous cell carcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP45
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C, do not freeze
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- Experimental details
- Immunohistochemical analysis of Phospho-S6 Ribosomal pSer240/244 using anti-Phospho-S6 Ribosomal pSer240/244 Monoclonal Antibody (Product # MA5-16398) in Breast Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.
