PA5-26041

antibody from Invitrogen Antibodies

Targeting: RPS6 S6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-26041

Provider

Invitrogen Antibodies

Product name

S6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, chicken, non-human primate, rat and Xenopus based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPS6 (arrow) using a RPS6 polyclonal antibody (Product # PA5-26041) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6 gene (Lane 2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a RPS6 polyclonal antibody (Product # PA5-26041) in WiDr cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a RPS6 polyclonal antibody (Product # PA5-26041) in mouse NIH-3T3 cell lysates (35 µg per lane).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human tonsil tissue using a RPS6 polyclonal antibody (Product # PA5-26041), followed by HRP-conjugated secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of WiDr cells using a RPS6 polyclonal antibody (Product # PA5-26041) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody