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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53742PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53742PU-N, RRID:AB_11147685
- Product name
- anti RPS6
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 227-257 amino acids from Human RPS6.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of RPS6 (arrow) using RPS6 Antibody(Ser240/244) Cat.-No AP53742PU-N. 293 cell lysates (2 ug/lane) either non-transfected (Lane 1) or transiently transfected with the RPS6 gene (Lane 2).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of RPS6 in WiDr cell line lysates (35ug/lane) using RPS6 Antibody(Ser240/244) Cat.-No AP53742PU-N. This demonstrates the RPS6 antibody detected the RPS6 protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of RPS6 in mouse NIH-3T3 cell line lysates (35ug/lane) using RPS6 Antibody(Ser240/244) Cat.-No AP53742PU-N. This demonstrates the RPS6 antibody detected the RPS6 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human tonsil tissue using RPS6 antibody (Ser240/244) Cat.-No AP53742PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of WiDr cells using RPS6 Antibody (Ser240/244) Cat.-No AP53742PU-N (right histogram) compared to a Negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
