Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 101-M34 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VEGFR-2/KDR
- Antibody type
- Monoclonal
- Antigen
- recombinant human soluble KDR (D1-7)
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCR
GQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLT
IPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSP
FIASVSDQHGVVYITENKNKTVVIPCLGSISNLNV
SLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISY
AGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLS
PSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPS
SKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTR
SDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGS
GMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGI
PLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPI
SKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGT
TQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAV
SVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEG
KNKTVSTLVIQAANVSALYKCEAVNKVGRGERVIS
FHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFE
NLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNA
TMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKT
KKRHCVVRQLTVLERVAPTITGNLENQTTSIGESI
EVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGN
RNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFII
EGA- Isotype
- IgG
- Antibody clone number
- (#4(20I6))
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Similarities Between Stem Cell Niches in Glioblastoma and Bone Marrow: Rays of Hope for Novel Treatment Strategies.
Similarities and differences of human and experimental mouse lymphangiomas
Hira VVV, Breznik B, Vittori M, Loncq de Jong A, Mlakar J, Oostra RJ, Khurshed M, Molenaar RJ, Lah T, Van Noorden CJF
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2020 Jan;68(1):33-57
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2020 Jan;68(1):33-57
Similarities and differences of human and experimental mouse lymphangiomas
Kasten P, Schnöink G, Bergmann A, Papoutsi M, Buttler K, Rössler J, Weich H, Wilting J
Developmental Dynamics 2007 October;236(10):2952-2961
Developmental Dynamics 2007 October;236(10):2952-2961
No comments: Submit comment
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Up-regulation of VEGFR-2 in primary HUVECs by bFGF. Freshly isolated HUVECs (passage 1) were cultured in EBM. Subconfluent cultures were stimulated with VEGF (5 ng/ml) or bFGF (10 ng/ml) for 3 days. Total lysate was prepared and subjected to immunoprecipitation (anti-human VEGFR-2 (Cl.3) [#101-M32] followed by Western blotting (anti-human VEGFR-2 (Cl.4) [#101-M34].
- Sample type
- HUVEC
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis of anti-human esKDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Left panel: monoclonal antibody against the soluble KDR D1-7); Right panel: polyclonal antibody (peptide) against against the unique C-terminal end of esKDR.
- Sample type
- Purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Up-regulation of VEGFR-2 in vein ECs of an intact human umbilical cord by bFGF. A fresh human umbilical cord was rinsed with PBS to remove residual blood cells, cut in small pieces (about 0.5 cm), incubated in EBM (1% FCS) and stimulated with or without 20 ng/ml bFGF for 24 h. Pieces were frozen in liquid nitrogen and used for immunohistochemistry using the mab anti-human VEGFR-2/Cl.4 (#101-M34) as detection antibody.
- Sample type
- Umbilical cord section
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis of VEGFR-2/KDR expression in HUVE cells [5µg/ml #101-M32; 5µg/ml PE goat anti-mouse IgG]. The experiment was performed by Trisha M. Westerhof, University of California, Irvine.
- Sample type
- HUVEC
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).