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- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [2]
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- Product number
- DM3522P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM3522P, RRID:AB_1008474
- Product name
- anti CD309 / VEGFR-2 / Flk-1
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human soluble extracellular KDR (D7) (110 kDa) protein (Cat.-No AR26018PU-N)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H3
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3: VEGFR-2/KDR Sandwich-ELISA using soluble KDR (D7) [Cat# S01-002] as standard. Mouse anti-human VEGFR-2 Cat.-No DM3522P was used as capture antibody, Biotinylated rabbit anti-human VEGFR-2 Cat.-No DP3509B was used for detection.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 5. Consecutive sections of unfixed, human foreskin. A) Staining with anti-soluble VEGFR2/KDR antibodies (Cat.-No AP26034PU-L). Note signal in epidermis and vessels. B) Staining with anti-membrane-bound VEGFR-2/KDR (Cat.-No DM3522P). Note staining in vessels. C) Negative control. Note non-specific fluorescence in the hornified layer of the epithelium. Provided by Prof. J. Wilting, Göttingen, Germany.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Up-regulation of VEGFR-2 in primary HUVECs by bFGF:Freshly isolated HUVECs (passage 1) were cultured in EBM. Subconfluent cultures were stimulated with VEGF (5 ng/ml) or bFGF (10 ng/ml) for 3 days. Total lysate was prepared and subjected to immunoprecipitation (anti-Human VEGFR-2 Cat.-No DM3522P) followed by Western blotting (anti-Human VEGFR-2 antibody Cat.-No DM3523P). (Bernhard Barleon et.al., unpublished data!)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 4. FACS analysis with primary HUVECs using anti-Human VEGFR-2 antibody Cat.-No DM3522P.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. FACS analysis of VEGFR-2/KDR expression in HUVE cells (upper level) and EPCs derived from PBMcs (lower level ) [5μg/ml DM3522P; 5μg/ml PE goat anti-mouse IgG]. The experiment was performed by Trisha M. Westerhof, University of California, Irvine.
