PA1-16612

antibody from Invitrogen Antibodies

Targeting: KDR CD309, FLK1, VEGFR, VEGFR2

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-16612

Provider

Invitrogen Antibodies

Product name

VEGF Receptor 1/2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The target sequence has 100% sequence homology with mouse and rat.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

4°C, do not freeze

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of VEGF Receptor 1/2 in formalin-fixed paraffin-embedded human breast carcinoma tissue. Samples were incubated in VEGF Receptor 1/2 polyclonal antibody (Product # PA1-16612) using a dilution of 1:500. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of VEGF Receptor 1/2 in formalin-fixed paraffin-embedded human breast carcinoma tissue section. Samples were incubated in VEGF Receptor 1/2 polyclonal antibody (Product # PA1-16612) using a dilution of 1:500. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells. The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity. Staining was performed by Histowiz.