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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-44307 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1orf77 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: LKQSLKQRLG KSNIQARLGR PIGALARGAI GGRGLPIIQR GLPRGGLRGG Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% PA5-44307 detects C1orf77 in IF application with mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of mouse brain cells using an anti-C1orf77 polyclonal antibody (Product # PA5-44307). Primary Antibody Dilution:1:500; Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody Dilution: 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C1orf77 (red) and Insulin (green) were performed on mouse pancreatic tissue from NOD (Non-obese diabetic) mice and CD1 (cluster of differentiation 1) mice. To expose target proteins, antigen retrieval was performed by microwaving tissues for three times with each 5 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval slides were wash with PBS and PBS+1% lamb serum, and then the slides were blocked with 1:50 normal donkey serum for 30 min at room temperature. The tissues were probed for C1orf77 (Product # PA5-44307) antibody 1:200 dilution and Insulin overnight at 4°C in a humidified chamber. Tissues were washed with PBS and PBS+1% lamb serum for 5 min and detection was performed using an fluorescence-conjugated secondary antibodies. Images were obtained using Zeiss confocal microscopy at 40X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of human brain stems (NT2) cells using an anti-C1orf77 polyclonal antibody (Product # PA5-44307). Primary Antibody Dilution: 1:500; Secondary Antibody: Goat anti-rabbit Alexa Fluor 594; Secondary Antibody Dilution: 1:1000.; LANE 2).
