PAB22197

antibody from Abnova Corporation

Targeting: CHTOP C1orf77, DKFZP547E1010, FOP, SRAG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22197

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22197, RRID:AB_10965584

Product name

CHTOP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CHTOP.

Antigen sequence

GRGRGMIGRGRGGFGGRGRGRGRGRGALARPVLTK
EQLDNQLDAYMSKTKGHLDAELDAYMAQTDPETND


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with CHTOP polyclonal antibody (Cat # PAB22197) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CHTOP polyclonal antibody (Cat # PAB22197) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with CHTOP polyclonal antibody (Cat # PAB22197) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)