Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12497-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12497-1-AP, RRID:AB_2260910
- Product name
- CRTC2,TORC2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CRTC2,TORC2 antibody (Cat. #12497-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Effect of chronic noise exposure on glucose and lipid metabolism in mice via modulating gut microbiota and regulating CREB/CRTC2 and SREBP1/SCD pathway.
CRTC2 activates the epithelial-mesenchymal transition of diabetic kidney disease through the CREB-Smad2/3 pathway.
LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation.
An insulin-regulated arrestin domain protein controls hepatic glucagon action.
Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.
Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis.
Gestational bisphenol A exposure impairs hepatic lipid metabolism by altering mTOR/CRTC2/SREBP1 in male rat offspring.
cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.
In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR.
SIK1 Regulates CRTC2-Mediated Gluconeogenesis Signaling Pathway in Human and Mouse Liver Cells.
The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.
Berberine Inhibits Gluconeogenesis in Skeletal Muscles and Adipose Tissues in Streptozotocin-induced Diabetic Rats via LKB1-AMPK-TORC2 Signaling Pathway.
Zhenqing recipe attenuates non-alcoholic fatty liver disease by regulating the SIK1/CRTC2 signaling in experimental diabetic rats.
CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.
Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats.
AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs.
Reduced growth hormone signaling and methionine restriction: interventions that improve metabolic health and extend life span.
CRTC2 activation in the suprachiasmatic nucleus, but not paraventricular nucleus, varies in a diurnal fashion and increases with nighttime light exposure.
Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.
Phorbol ester-induced human cytomegalovirus major immediate-early (MIE) enhancer activation through PKC-delta, CREB, and NF-kappaB desilences MIE gene expression in quiescently infected human pluripotent NTera2 cells.
Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells.
Wu S, Du W, Wu Z, Wen F, Zhong X, Huang X, Gu H, Wang J
Ecotoxicology and environmental safety 2024 Jan 15;270:115887
Ecotoxicology and environmental safety 2024 Jan 15;270:115887
CRTC2 activates the epithelial-mesenchymal transition of diabetic kidney disease through the CREB-Smad2/3 pathway.
Li Y, Zhang Y, Shi H, Liu X, Li Z, Zhang J, Wang X, Wang W, Tong X
Molecular medicine (Cambridge, Mass.) 2023 Oct 26;29(1):146
Molecular medicine (Cambridge, Mass.) 2023 Oct 26;29(1):146
LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation.
Compton SE, Kitchen-Goosen SM, DeCamp LM, Lau KH, Mabvakure B, Vos M, Williams KS, Wong KK, Shi X, Rothbart SB, Krawczyk CM, Jones RG
Molecular cell 2023 Jun 1;83(11):1872-1886.e5
Molecular cell 2023 Jun 1;83(11):1872-1886.e5
An insulin-regulated arrestin domain protein controls hepatic glucagon action.
Dagdeviren S, Hoang MF, Sarikhani M, Meier V, Benoit JC, Okawa MC, Melnik VY, Ricci-Blair EM, Foot N, Friedline RH, Hu X, Tauer LA, Srinivasan A, Prigozhin MB, Shenoy SK, Kumar S, Kim JK, Lee RT
The Journal of biological chemistry 2023 Aug;299(8):105045
The Journal of biological chemistry 2023 Aug;299(8):105045
Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.
He L, Zhou X, Wu Z, Feng Y, Liu D, Li T, Yin Y
Animal nutrition (Zhongguo xu mu shou yi xue hui) 2022 Sep;10:137-147
Animal nutrition (Zhongguo xu mu shou yi xue hui) 2022 Sep;10:137-147
Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis.
Zhang J, Chi R, Zhang Y, Xie Y, Liu Y, Bao Q, Lv H, Han B, Sun H, Sun P
Nutrition & metabolism 2022 Nov 29;19(1):78
Nutrition & metabolism 2022 Nov 29;19(1):78
Gestational bisphenol A exposure impairs hepatic lipid metabolism by altering mTOR/CRTC2/SREBP1 in male rat offspring.
Yang Q, Mao Y, Wang J, Yu H, Zhang X, Pei X, Duan Z, Xiao C, Ma M
Human & experimental toxicology 2022 Jan-Dec;41:9603271221129852
Human & experimental toxicology 2022 Jan-Dec;41:9603271221129852
cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.
Mi Z, Song Y, Wang J, Liu Z, Cao X, Dang L, Lu Y, Sun Y, Xiong H, Zhang L, Chen Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Apr;9(10):e2104578
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Apr;9(10):e2104578
In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR.
Charbord J, Ren L, Sharma RB, Johansson A, Ågren R, Chu L, Tworus D, Schulz N, Charbord P, Stewart AF, Wang P, Alonso LC, Andersson O
Nature metabolism 2021 May;3(5):682-700
Nature metabolism 2021 May;3(5):682-700
SIK1 Regulates CRTC2-Mediated Gluconeogenesis Signaling Pathway in Human and Mouse Liver Cells.
Wang C, Song D, Fu J, Wen X
Frontiers in endocrinology 2020;11:580
Frontiers in endocrinology 2020;11:580
The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.
Ling Q, Huang H, Han Y, Zhang C, Zhang X, Chen K, Wu L, Tang R, Zheng Z, Zheng S, Li L, Wang B
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2020 Mar;20(3):701-713
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2020 Mar;20(3):701-713
Berberine Inhibits Gluconeogenesis in Skeletal Muscles and Adipose Tissues in Streptozotocin-induced Diabetic Rats via LKB1-AMPK-TORC2 Signaling Pathway.
Xu XH, Hu Q, Zhou LS, Xu LJ, Zou X, Lu FE, Yi P
Current medical science 2020 Jun;40(3):530-538
Current medical science 2020 Jun;40(3):530-538
Zhenqing recipe attenuates non-alcoholic fatty liver disease by regulating the SIK1/CRTC2 signaling in experimental diabetic rats.
Song D, Yin L, Wang C, Wen X
BMC complementary medicine and therapies 2020 Jan 31;20(1):27
BMC complementary medicine and therapies 2020 Jan 31;20(1):27
CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.
Kanki H, Sasaki T, Matsumura S, Kawano T, Todo K, Okazaki S, Nishiyama K, Takemori H, Mochizuki H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Dec 2;40(49):9533-9546
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Dec 2;40(49):9533-9546
Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats.
Song D, Yin L, Wang C, Wen X
PloS one 2019;14(6):e0210930
PloS one 2019;14(6):e0210930
AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs.
He L, Huang N, Li H, Tian J, Zhou X, Li T, Yao K, Wu G, Yin Y
Journal of agricultural and food chemistry 2017 Mar 22;65(11):2287-2298
Journal of agricultural and food chemistry 2017 Mar 22;65(11):2287-2298
Reduced growth hormone signaling and methionine restriction: interventions that improve metabolic health and extend life span.
Brown-Borg HM
Annals of the New York Academy of Sciences 2016 Jan;1363:40-9
Annals of the New York Academy of Sciences 2016 Jan;1363:40-9
CRTC2 activation in the suprachiasmatic nucleus, but not paraventricular nucleus, varies in a diurnal fashion and increases with nighttime light exposure.
Highland JA, Weiser MJ, Hinds LR, Spencer RL
American journal of physiology. Cell physiology 2014 Oct 1;307(7):C611-21
American journal of physiology. Cell physiology 2014 Oct 1;307(7):C611-21
Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.
Sakamoto K, Norona FE, Alzate-Correa D, Scarberry D, Hoyt KR, Obrietan K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 22;33(21):9021-7
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 22;33(21):9021-7
Phorbol ester-induced human cytomegalovirus major immediate-early (MIE) enhancer activation through PKC-delta, CREB, and NF-kappaB desilences MIE gene expression in quiescently infected human pluripotent NTera2 cells.
Liu X, Yuan J, Wu AW, McGonagill PW, Galle CS, Meier JL
Journal of virology 2010 Sep;84(17):8495-508
Journal of virology 2010 Sep;84(17):8495-508
Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells.
Yuan J, Liu X, Wu AW, McGonagill PW, Keller MJ, Galle CS, Meier JL
Journal of virology 2009 Jul;83(13):6391-403
Journal of virology 2009 Jul;83(13):6391-403
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP(CRTC2,TORC2 antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 12497-1-AP(CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
