12497-1-AP

antibody from Proteintech Group

Targeting: CRTC2 TORC2

Provider product page for 12497-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [29]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 12497-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12497-1-AP, RRID:AB_2260910
Product name: CRTC2,TORC2 antibody
Antibody type: Polyclonal
Description: KD/KO validated CRTC2,TORC2 antibody (Cat. #12497-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CRTC2 protein structure - 12497-1-AP shown in red.

Submitted references PBX1 Improves Cognition and Reduces Amyloid-β Pathology in APP/PS1 Mice by Transcriptionally Activating the CRTC2-CREB Pathway.

Liu Z, Meng X, Lu R, Meng X, Li S, Wang Y, Liu X, Liu X, Liu J
Aging cell 2026 Jan;25(1):e70311

Construction of 3D Enhanced CT Radiomics-Based Model to Predict Circadian Gene Expression and Prognosis in Hepatocellular Carcinoma.

Zhao J, Zhou H, Wang C, Zhang W, Pang Y, Zheng H, Zhang L, Zhou J, Hu Z
Journal of hepatocellular carcinoma 2025;12:2989-3009

Fas apoptotic inhibitor molecule 2 mitigates metabolic dysfunction-associated fatty liver disease through autophagic CRTC2 degradation.

Yu Y, Hu S, Zhang T, Shi H, Li D, Huang Y, Zhang Y, Wang H, Hu Y, Yu H, Zhao GN, Zhang P
Experimental & molecular medicine 2025 Oct;57(10):2331-2343

CRTC2 Forms Co-Condensates with YTHDF2 That Enhance Translational Efficiency of m6A-Modified mRNAs to Drive Hepatocarcinogenesis and Lenvatinib Resistance.

Wang M, Zou F, Wang S, Yang Y, Xia C, Chen L, Liu B, Li L, Li MJ, Li H, Song W, Niu R, Yuan Z, Yang J, Li X, Chen K, Wu Z, Mi Z
Cancer research 2025 Jun 2;85(11):2046-2066

CRTC3 restricts SARS-CoV-2 replication and is antagonized by CREB.

Yang L, Zeng XT, Luo RH, Tang Y, Ren SX, Long XY, Fu XH, Zhang WJ, Ren HY, Zheng YT, Cheng W
Virologica Sinica 2025 Feb;40(1):92-108

Hypoxia Promotes Gluconeogenesis Through PGC-1α in the Liver of Lasiopodomys brandtii.

Li M, Huang M, Wan J, Gao Y, Song J, Li X, Li Q, Cheng H, Shao T, Wang Z
Integrative zoology 2025 Feb 28;

Piperlongumine, a natural alkaloid from Piper longum L. ameliorates metabolic-associated fatty liver disease by antagonizing the thromboxane A(2) receptor.

Dai Y, Chen J, Fang J, Liang S, Zhang H, Li H, Chen W
Biochemical pharmacology 2024 Nov;229:116518

Hypomethylation-enhanced CRTC2 expression drives malignant phenotypes and primary resistance to immunotherapy in hepatocellular carcinoma.

Zhang R, Dai J, Yao F, Zhou S, Huang W, Xu J, Yu K, Chen Y, Fan B, Zhang L, Xu J, Li Q
iScience 2024 Jun 21;27(6):109821

Effect of chronic noise exposure on glucose and lipid metabolism in mice via modulating gut microbiota and regulating CREB/CRTC2 and SREBP1/SCD pathway.

Wu S, Du W, Wu Z, Wen F, Zhong X, Huang X, Gu H, Wang J
Ecotoxicology and environmental safety 2024 Jan 15;270:115887

CRTC2 activates the epithelial-mesenchymal transition of diabetic kidney disease through the CREB-Smad2/3 pathway.

Li Y, Zhang Y, Shi H, Liu X, Li Z, Zhang J, Wang X, Wang W, Tong X
Molecular medicine (Cambridge, Mass.) 2023 Oct 26;29(1):146

LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation.

Compton SE, Kitchen-Goosen SM, DeCamp LM, Lau KH, Mabvakure B, Vos M, Williams KS, Wong KK, Shi X, Rothbart SB, Krawczyk CM, Jones RG
Molecular cell 2023 Jun 1;83(11):1872-1886.e5

An insulin-regulated arrestin domain protein controls hepatic glucagon action.

Dagdeviren S, Hoang MF, Sarikhani M, Meier V, Benoit JC, Okawa MC, Melnik VY, Ricci-Blair EM, Foot N, Friedline RH, Hu X, Tauer LA, Srinivasan A, Prigozhin MB, Shenoy SK, Kumar S, Kim JK, Lee RT
The Journal of biological chemistry 2023 Aug;299(8):105045

Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.

He L, Zhou X, Wu Z, Feng Y, Liu D, Li T, Yin Y
Animal nutrition (Zhongguo xu mu shou yi xue hui) 2022 Sep;10:137-147

Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis.

Zhang J, Chi R, Zhang Y, Xie Y, Liu Y, Bao Q, Lv H, Han B, Sun H, Sun P
Nutrition & metabolism 2022 Nov 29;19(1):78

Gestational bisphenol A exposure impairs hepatic lipid metabolism by altering mTOR/CRTC2/SREBP1 in male rat offspring.

Yang Q, Mao Y, Wang J, Yu H, Zhang X, Pei X, Duan Z, Xiao C, Ma M
Human & experimental toxicology 2022 Jan-Dec;41:9603271221129852

cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

Mi Z, Song Y, Wang J, Liu Z, Cao X, Dang L, Lu Y, Sun Y, Xiong H, Zhang L, Chen Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Apr;9(10):e2104578

In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR.

Charbord J, Ren L, Sharma RB, Johansson A, Ågren R, Chu L, Tworus D, Schulz N, Charbord P, Stewart AF, Wang P, Alonso LC, Andersson O
Nature metabolism 2021 May;3(5):682-700

SIK1 Regulates CRTC2-Mediated Gluconeogenesis Signaling Pathway in Human and Mouse Liver Cells.

Wang C, Song D, Fu J, Wen X
Frontiers in endocrinology 2020;11:580

The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.

Ling Q, Huang H, Han Y, Zhang C, Zhang X, Chen K, Wu L, Tang R, Zheng Z, Zheng S, Li L, Wang B
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2020 Mar;20(3):701-713

Berberine Inhibits Gluconeogenesis in Skeletal Muscles and Adipose Tissues in Streptozotocin-induced Diabetic Rats via LKB1-AMPK-TORC2 Signaling Pathway.

Xu XH, Hu Q, Zhou LS, Xu LJ, Zou X, Lu FE, Yi P
Current medical science 2020 Jun;40(3):530-538

Zhenqing recipe attenuates non-alcoholic fatty liver disease by regulating the SIK1/CRTC2 signaling in experimental diabetic rats.

Song D, Yin L, Wang C, Wen X
BMC complementary medicine and therapies 2020 Jan 31;20(1):27

CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.

Kanki H, Sasaki T, Matsumura S, Kawano T, Todo K, Okazaki S, Nishiyama K, Takemori H, Mochizuki H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Dec 2;40(49):9533-9546

Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats.

Song D, Yin L, Wang C, Wen X
PloS one 2019;14(6):e0210930

AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs.

He L, Huang N, Li H, Tian J, Zhou X, Li T, Yao K, Wu G, Yin Y
Journal of agricultural and food chemistry 2017 Mar 22;65(11):2287-2298

Reduced growth hormone signaling and methionine restriction: interventions that improve metabolic health and extend life span.

Brown-Borg HM
Annals of the New York Academy of Sciences 2016 Jan;1363:40-9

CRTC2 activation in the suprachiasmatic nucleus, but not paraventricular nucleus, varies in a diurnal fashion and increases with nighttime light exposure.

Highland JA, Weiser MJ, Hinds LR, Spencer RL
American journal of physiology. Cell physiology 2014 Oct 1;307(7):C611-21

Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.

Sakamoto K, Norona FE, Alzate-Correa D, Scarberry D, Hoyt KR, Obrietan K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 22;33(21):9021-7

Phorbol ester-induced human cytomegalovirus major immediate-early (MIE) enhancer activation through PKC-delta, CREB, and NF-kappaB desilences MIE gene expression in quiescently infected human pluripotent NTera2 cells.

Liu X, Yuan J, Wu AW, McGonagill PW, Galle CS, Meier JL
Journal of virology 2010 Sep;84(17):8495-508

Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells.

Yuan J, Liu X, Wu AW, McGonagill PW, Keller MJ, Galle CS, Meier JL
Journal of virology 2009 Jul;83(13):6391-403

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP(CRTC2,TORC2 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human gliomas using 12497-1-AP(CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue