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- Antibody Data
- Antigen structure
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- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-15710 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Torc2 Monoclonal Antibody (5B10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15710 targets CRTC2 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples. The MA5-15710 immunogen is purified recombinant fragment of human CRTC2 expressed in E. Coli. MA5-15710 detects CRTC2 which has a predicted molecular weight of approximately 80kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5B10
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Function and Transcriptional Regulation of Bovine TORC2 Gene in Adipocytes: Roles of C/EBP, XBP1, INSM1 and ZNF263.
Khan R, Raza SHA, Junjvlieke Z, Xiaoyu W, Garcia M, Elnour IE, Hongbao W, Linsen Z
International journal of molecular sciences 2019 Sep 4;20(18)
International journal of molecular sciences 2019 Sep 4;20(18)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CRTC2 monoclonal antibody (Product # MA5-15710) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CRTC2 monoclonal antibody (Product # MA5-15710) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using CRTC2 monoclonal antibody (Product # MA5-15710) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Transfection efficiency, tissue and cellular expression and sub cellular localization of the transducer of regulated cAMP response element-binding protein (CREB) 2 ( TORC2 ) gene. ( A ) The mRNA expression level of the TORC2 gene in different tissues. Glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) was used as the housing gene ( B , C ) Real time qPCR was used for the detection of the TORC2 gene in preadipocytes transfected with OE-NC, OE-TORC2, siNC, and siTORC2. ( D ) Western blot analysis of the TORC2 protein gene in adipocytes transfected with OE-NC, OE-TORC2, siNC, and siTORC2. The analysis in ( D ) shows the overexpression efficiency of OE-TORC2 compared with OE-NC, and the silencing efficiency of TORC2 after transfecting the siTORC2 compared with siNC. ( E ) Subcellular localization of TORC2, immunofluorescence with the anti-TORC2 antibody (red), and nuclei were visualized with DAPI (blue) in bovine adipocytes. The right panel shows merged views. Pictures were captured though an Olympus IX71 microscope (OLYMPUS, Tokyo, Japan). One-way ANOVA and t-test were used for statistical analysis. Asterisks indicate significant variations. **** p < 0.0012.2. TORC2 promotes preadipocyte proliferation.
