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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-15710 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Torc2 Monoclonal Antibody (5B10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15710 targets CRTC2 in FACS, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.
- Antibody clone number
- 5B10
- Concentration
- Conc. Not Determined
Submitted references Function and Transcriptional Regulation of Bovine TORC2 Gene in Adipocytes: Roles of C/EBP, XBP1, INSM1 and ZNF263.
Khan R, Raza SHA, Junjvlieke Z, Xiaoyu W, Garcia M, Elnour IE, Hongbao W, Linsen Z
International journal of molecular sciences 2019 Sep 4;20(18)
International journal of molecular sciences 2019 Sep 4;20(18)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRTC2 using CRTC2 monoclonal antibody (Product # MA5-15710) in HeLa (1) and HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRTC2 using CRTC2 monoclonal antibody (Product # MA5-15710) in HeLa (1) and HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CRTC2 monoclonal antibody (Product # MA5-15710) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CRTC2 monoclonal antibody (Product # MA5-15710) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using CRTC2 monoclonal antibody (Product # MA5-15710) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using CRTC2 monoclonal antibody (Product # MA5-15710) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Transfection efficiency, tissue and cellular expression and sub cellular localization of the transducer of regulated cAMP response element-binding protein (CREB) 2 ( TORC2 ) gene. ( A ) The mRNA expression level of the TORC2 gene in different tissues. Glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) was used as the housing gene ( B , C ) Real time qPCR was used for the detection of the TORC2 gene in preadipocytes transfected with OE-NC, OE-TORC2, siNC, and siTORC2. ( D ) Western blot analysis of the TORC2 protein gene in adipocytes transfected with OE-NC, OE-TORC2, siNC, and siTORC2. The analysis in ( D ) shows the overexpression efficiency of OE-TORC2 compared with OE-NC, and the silencing efficiency of TORC2 after transfecting the siTORC2 compared with siNC. ( E ) Subcellular localization of TORC2, immunofluorescence with the anti-TORC2 antibody (red), and nuclei were visualized with DAPI (blue) in bovine adipocytes. The right panel shows merged views. Pictures were captured though an Olympus IX71 microscope (OLYMPUS, Tokyo, Japan). One-way ANOVA and t-test were used for statistical analysis. Asterisks indicate significant variations. **** p < 0.0012.2. TORC2 promotes preadipocyte proliferation.
