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LearnHPA027305
antibody from Atlas Antibodies
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- HPA027305 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA027305, RRID:AB_1854429
- Product name
- Anti-NFKB1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NFKB1, Gene description: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Alternative Gene Names: KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50, Validated applications: WB, IHC, ICC, ChIP, Uniprot ID: P19838, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Multiple Nf1 Schwann cell populations reprogram the plexiform neurofibroma tumor microenvironment
Defining the structure of the NF-ĸB pathway in human immune cells using quantitative proteomic data
MicroRNA Sequencing Analysis in Obstructive Sleep Apnea and Depression: Anti-Oxidant and MAOA-Inhibiting Effects of miR-15b-5p and miR-92b-3p through Targeting PTGS1-NF-κB-SP1 Signaling
Cancer-associated mesenchymal stroma fosters the stemness of osteosarcoma cells in response to intratumoral acidosis via NF-κB activation
Kershner L, Choi K, Wu J, Zhang X, Perrino M, Salomonis N, Shern J, Ratner N
JCI Insight 2022;7(18)
JCI Insight 2022;7(18)
Defining the structure of the NF-ĸB pathway in human immune cells using quantitative proteomic data
Kok F, Wang H, Riedlova P, Goodyear C, Carmody R
Cellular Signalling 2021;88
Cellular Signalling 2021;88
MicroRNA Sequencing Analysis in Obstructive Sleep Apnea and Depression: Anti-Oxidant and MAOA-Inhibiting Effects of miR-15b-5p and miR-92b-3p through Targeting PTGS1-NF-κB-SP1 Signaling
Chen Y, Hsu P, Su M, Chen T, Hsiao C, Chin C, Liou C, Wang P, Wang T, Lin Y, Lee C, Lin M
Antioxidants 2021;10(11):1854
Antioxidants 2021;10(11):1854
Cancer-associated mesenchymal stroma fosters the stemness of osteosarcoma cells in response to intratumoral acidosis via NF-κB activation
Avnet S, Di Pompo G, Chano T, Errani C, Ibrahim-Hashim A, Gillies R, Donati D, Baldini N
International Journal of Cancer 2017;140(6):1331-1345
International Journal of Cancer 2017;140(6):1331-1345
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NFKB1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human lymph node and cerebral cortex tissues using HPA027305 antibody. Corresponding NFKB1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-NFKB1 (HPA027305, Lot 000014987) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
