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LearnMAB10830
antibody from Abnova Corporation
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10830 - Provider product page
- Provider
- Abnova Corporation
- Product name
- NFKB1 monoclonal antibody, clone 5D10D11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NFKB1.
- Isotype
- IgG
- Antibody clone number
- 5D10D11
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) against K-562 (1), Jurkat (2), A-431 (3), HeLa (4), THP-1 (5) and MCF-7 (6) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of U251 cells using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human intima cancer tissues (A) and human bladder cancer tissues (B) using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) (green) and negative control (purple).
- Validation comment
- Flow Cytometry
