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LearnARG51521
antibody from Arigo
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG51521 - Provider product page
- Provider
- Arigo
- Product name
- anti-NFkB p105/p50 phospho (Ser337) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of serine 337(R-K-S(p)-D-L) derived from Human NFκB-p105/p50.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extract from HeLa cells, stained with anti-NFkB p105/p50 (phospho Ser337) antibody ARG51521, Lane 1 and 2).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from HeLa cells, treated with Noc or calf intestinal phosphatase (CIP), stained with anti-NFkB p105/p50 (phospho Ser337) antibody ARG51521.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: methanol-fixed HeLa cells stained with anti-NFkB p105/p50 (phospho Ser337) antibody ARG51521, Red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin- embedded human breast carcinoma tissue stained with anti-NFkB p105/p50 (phospho Ser337) antibody ARG51521).
