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LearnR31569
antibody from NSJ Bioreagents
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- R31569 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- p50 Antibody / p105 / NFKB1
- Antibody type
- Polyclonal
- Description
- This highly specific p50 antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- The p50 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeat freeze/thaws.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of p50 antibody and 1: rat spleen; 2: (r) PC-12; and human samples 3: HeLa; 4: A431; 5: Jurkat; 6: MCF-7; Predicted/observed size: 50/105KD depending on sample
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of p50 antibody and recombinant human protein (0.5ng)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Raji, 2) SH-SY5Y, 3) HeLa and 4) Jurkat cell lysate with p50 antibody. Expected molecular weight: 50 kDa / 105 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with p50 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: p50 antibody testing of human lung cancer tissue
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with p50 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p50 antibody.
