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LearnAM06646SU-N
antibody from OriGene
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AM06646SU-N - Provider product page
- Provider
- OriGene
- Product name
- NFKB1 mouse monoclonal antibody, clone 5D10D11, Ascites
- Antibody type
- Monoclonal
- Description
- NFKB1 mouse monoclonal antibody, clone 5D10D11, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NFKB1
- Isotype
- IgG
- Antibody clone number
- 5D10D11
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using NFKB1 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of U251 cells using NFKB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human intima cancer tissues (left) and human bladder cancer tissues (right) using NFKB1 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using NFKB1 mouse mAb (green) and negative control (purple).
- Validation comment
- FC
