14-6732-81
antibody from Invitrogen Antibodies
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Other assay [2]
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- Product number
- 14-6732-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFkB p50/p105 Polyclonal Antibody, eBioscience™
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Description: The polyclonal antibody reacts with mouse, rat, and human NFkB p50; the antibody was raised against a peptide mapping to the carboxy terminus of human NFkB p50. Members of the rel/NFkB family of transcription factors are involved in the regulation of cellular responses, such as growth, development, and the inflammatory response. They share a structural motif known as the rel homology region (RHR), the C-terminal one third of which mediates protein dimerization (2, 6, 8). Complexes of p50 (NF-kB1) or p52 (NF-kB2) are generated through the processing of p105 and p100 precursors, respectively. These are usually associated with members of the Rel family (p65, c-Rel, Rel B). The homo- and heterodimer formed through combinations of NFkB/Rel proteins bind distinct kB sites to regulate the transcription of different genes (7, 9). In resting cells, NFkB is retained in the cytoplasm bound to inhibitory proteins of the IkB family. Degradation of IkB proteins occurs with cell activation, via of variety of signals, including inflammatory cytokines and bacterial lipopolysaccharides (LPS) as well as oxidative and fluid mechanical stress. This results in nuclear translocation of NFkB and the transcriptional gene activation of proinflammatory genes (1, 9). It has been suggested that NFkB plays a role in the development of numerous pathological states. Activation of NFkB induces gene programs leading to transcription of factors that promote inflammation, such as leukocyte adhesion molecules, cytokines, and chemokines. It is also thought that there are some substances with possible anti-inflammatory effects that are also NFkB regulated. There is some evidence indicating NFkB as a key factor in the pathophysiology of cardiac ischemia-reperfusion injury as well as the development of insulin dependent Diabetes Mellitus (4, 3). Applications Reported: Purified anti-mo/hu/rat NFkB p50 and p105 poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining. Applications Tested: Purified anti-mo/hu/rat NFkB p50 and p105 poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Inhibition of TNF-α Restores Muscle Force, Inhibits Inflammation, and Reduces Apoptosis of Traumatized Skeletal Muscles.
Inhibitory effects of quail egg on mast cells degranulation by suppressing PAR2-mediated MAPK and NF-kB activation.
Simultaneous inhibition of FXR and TGR5 exacerbates atherosclerotic formation.
Th9 Cells Represent a Unique Subset of CD4(+) T Cells Endowed with the Ability to Eradicate Advanced Tumors.
Cutting Edge: Synchronization of IRF1, JunB, and C/EBPβ Activities during TLR3-TLR7 Cross-Talk Orchestrates Timely Cytokine Synergy in the Proinflammatory Response.
NF-kappaB activation through the alternative pathway correlates with chemoresistance and poor survival in extranodal NK/T-cell lymphoma, nasal type.
Nuclear factor kappa-B and the heart.
The NFkappaBb/IkappaB system in acute inflammation.
NF-kappaB and the innate immune response.
The ubiquitin-dependent proteolytic system and other potential targets for the modulation of nuclear factor-kB (NF-kB).
Construction, expression, purification and functional analysis of recombinant NFkappaB p50/p65 heterodimer.
Characterization of the dimer interface of transcription factor NFkappaB p50 homodimer.
Antioxidants, NFkappaB activation, and diabetogenesis.
The role of DNA in the mechanism of NFkappaB dimer formation: crystal structures of the dimerization domains of the p50 and p65 subunits.
Stratos I, Behrendt AK, Anselm C, Gonzalez A, Mittlmeier T, Vollmar B
Cells 2022 Aug 3;11(15)
Cells 2022 Aug 3;11(15)
Inhibitory effects of quail egg on mast cells degranulation by suppressing PAR2-mediated MAPK and NF-kB activation.
Lianto P, Ogutu FO, Zhang Y, He F, Che H
Food & nutrition research 2018;62
Food & nutrition research 2018;62
Simultaneous inhibition of FXR and TGR5 exacerbates atherosclerotic formation.
Miyazaki-Anzai S, Masuda M, Kohno S, Levi M, Shiozaki Y, Keenan AL, Miyazaki M
Journal of lipid research 2018 Sep;59(9):1709-1713
Journal of lipid research 2018 Sep;59(9):1709-1713
Th9 Cells Represent a Unique Subset of CD4(+) T Cells Endowed with the Ability to Eradicate Advanced Tumors.
Lu Y, Wang Q, Xue G, Bi E, Ma X, Wang A, Qian J, Dong C, Yi Q
Cancer cell 2018 Jun 11;33(6):1048-1060.e7
Cancer cell 2018 Jun 11;33(6):1048-1060.e7
Cutting Edge: Synchronization of IRF1, JunB, and C/EBPβ Activities during TLR3-TLR7 Cross-Talk Orchestrates Timely Cytokine Synergy in the Proinflammatory Response.
Liu Q, Zhu Y, Yong WK, Sze NS, Tan NS, Ding JL
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 1;195(3):801-5
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 1;195(3):801-5
NF-kappaB activation through the alternative pathway correlates with chemoresistance and poor survival in extranodal NK/T-cell lymphoma, nasal type.
Liu X, Wang B, Ma X, Guo Y
Japanese journal of clinical oncology 2009 Jul;39(7):418-24
Japanese journal of clinical oncology 2009 Jul;39(7):418-24
Nuclear factor kappa-B and the heart.
Valen G, Yan ZQ, Hansson GK
Journal of the American College of Cardiology 2001 Aug;38(2):307-14
Journal of the American College of Cardiology 2001 Aug;38(2):307-14
The NFkappaBb/IkappaB system in acute inflammation.
Lentsch AB, Ward PA
Archivum immunologiae et therapiae experimentalis 2000;48(2):59-63
Archivum immunologiae et therapiae experimentalis 2000;48(2):59-63
NF-kappaB and the innate immune response.
Hatada EN, Krappmann D, Scheidereit C
Current opinion in immunology 2000 Feb;12(1):52-8
Current opinion in immunology 2000 Feb;12(1):52-8
The ubiquitin-dependent proteolytic system and other potential targets for the modulation of nuclear factor-kB (NF-kB).
Magnani M, Crinelli R, Bianchi M, Antonelli A
Current drug targets 2000 Dec;1(4):387-99
Current drug targets 2000 Dec;1(4):387-99
Construction, expression, purification and functional analysis of recombinant NFkappaB p50/p65 heterodimer.
Chen FE, Kempiak S, Huang DB, Phelps C, Ghosh G
Protein engineering 1999 May;12(5):423-8
Protein engineering 1999 May;12(5):423-8
Characterization of the dimer interface of transcription factor NFkappaB p50 homodimer.
Sengchanthalangsy LL, Datta S, Huang DB, Anderson E, Braswell EH, Ghosh G
Journal of molecular biology 1999 Jun 18;289(4):1029-40
Journal of molecular biology 1999 Jun 18;289(4):1029-40
Antioxidants, NFkappaB activation, and diabetogenesis.
Ho E, Bray TM
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) 1999 Dec;222(3):205-13
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) 1999 Dec;222(3):205-13
The role of DNA in the mechanism of NFkappaB dimer formation: crystal structures of the dimerization domains of the p50 and p65 subunits.
Huang DB, Huxford T, Chen YQ, Ghosh G
Structure (London, England : 1993) 1997 Nov 15;5(11):1427-36
Structure (London, England : 1993) 1997 Nov 15;5(11):1427-36
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 The effect of QE on the PAR-2-mediated MAPK and NF-kB cell signaling pathways of HMC-1 cells. (a) mRNA fold induction of PAR-2. (b) Western blotting of MAPK signaling. MAPK fold activation data analysis: (c) pERK1/2, (d) p-p38 and (e) p-JNK1/2. (f) Western blotting of NF-kB signaling. NF-kB fold activation data analysis: (g) IKK-alpha, (h) NF-kB p50, and (i) NF-kB p65. Results are expressed as mean +- SEM. * p < 0.05 as compared to the control group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Representative Western blot of TRAF-2, NF-kappaB subunits p105 and p65, IL-6 and TNF-alpha per GAPDH protein in the injured soleus muscle tissue. Animals received either infliximab (IFX) or NaCl (CTRL) directly after the soft tissue injury. Measurement was conducted at days one, four, and 14.