PA5-17150

antibody from Invitrogen Antibodies

Targeting: NFKB1 KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17150

Provider

Invitrogen Antibodies

Product name

NFkB p105 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with the p50 subunit or other NF--kappaB family members.

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

30 µg/mL

Storage

-20°C

References

Submitted references

A central role of IKK2 and TPL2 in JNK activation and viral B-cell transformation.

Voigt S, Sterz KR, Giehler F, Mohr AW, Wilson JB, Moosmann A, Kieser A
Nature communications 2020 Feb 4;11(1):685

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF-kappa-B1 p105 in extracts from THP-1 cells, differentiated with TPA (80 nM for 24 h) and treated with 1 µg/mL LPS, using NF-kappa-B1 p105 polyclonal antibody (Product # PA5-17150).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 LMP1 activates TPL2 via IKK2. a , b LMP1 activity induces TPL2 phosphorylation at serine 400. a HEK293 cells were transfected with TPL2-myc together with HA-LMP1 wildtype or inactive AAA/Y384G. Phosphorylation of immunoprecipitated TPL2-myc was detected with the P-TPL2 S400 antibody. b HEK293 cells, which stably express NGFR-LMP1, were transfected with Myc-tagged TPL2. NGFR-LMP1 activity was induced by antibody crosslinking 24 h after transfection. TPL2-myc was immunoprecipitated and TPL2-myc phosphorylation was detected. c LMP1 activity causes TPL2 degradation. Immunoblot analysis of wtMEF:NGFR-LMP1wt cells induced by antibody crosslinking. d TPL2 is released from its complex with p105 after LMP1 induction. HEK293:NGFR-LMP1wt cells were transfected with Flag-p105 and TPL2-myc vectors. After 24 h, NGFR-LMP1 was induced for the indicated times and Flag-p105 was precipitated via its Flag-tag. Interaction of TPL2 and p105 was analysed using the indicated antibodies. e The knockdown of IKK2 blocks LMP1-induced TPL2 degradation. Cells were transfected twice with IKK2 siRNA (siIKK2) or non-targeting control siRNA (siCTRL). After 24 h, NGFR-LMP1 activity was induced and TPL2, IkappaBalpha, and IKK2 levels were analysed. f LMP1-induced TPL2 degradation depends on IKK activity. NGFR-LMP1 was induced in MEFs by antibody crosslinking in the presence of DMSO or 5 uM ACHP. g LMP1 fails to induce TPL2 degradation in the absence of IKK2. NGFR-LMP1 activity was induced in wildtype and