MA5-24735

antibody from Invitrogen Antibodies

Targeting: PPP2R1B PP2A-Abeta, PR65B

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24735

Provider

Invitrogen Antibodies

Product name

Anti-PPP2R1B Monoclonal Antibody (1496CT356.164.25.226)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1496CT356.164.25.226

Vial size

400 µl

Concentration

0.68 mg/ml

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PPP2R1B in U-2 OS cells. Fixation was performed with 4% paraformaldehyde and the cells were permeabilized with 0.1% Triton X-100. Sample was probed with a PPP2R1B monoclonal antibody (Product # MA5-24735) using a dilution of 1:25, followed by Dylight 488-conjugated goat anti-mouse IgG using a dilution of 1:200. Dylight 554 (1:100) and DAPI were used to counterstain cytoplasmic actin and cell nuclei, respectively.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human spleen tissue. Fixation was performed with formaldehyde, blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Sample was probed for 1 hours at 37 C with a PPP2R1B monoclonal antibody (Product # ) with a dilution of 1:25. Detection was performed with polyvalent goat biotinylated secondary antibody.