Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-93159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP2R1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YAIREAATNN LMKLVQKFGT EWAQNTIVPK VLVMANDPNY LHRMTTLFCI NALSEACGQE ITTKQMLPIV LKMAGDQVAN VRFNVAKSLQ KIGPILDTNA LQGEVKPVLQ KLGQDEDMDV KYFAQEAISV VAQRLRKLEF PVKDSGEPSV PRADKNHFPR PTVPGEDMGK GPVYQLRGDT RDTLAQLGIA ELVHFSQSTD; Positive Samples: Jurkat
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.71 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of Jurkat cells, using PPP2R1B Polyclonal antibody (Product # PA5-93159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PPP2R1B in extracts of Jurkat cells using PPP2R1B Polyclonal Antibody (Product # PA5-93159) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PPP2R1B was performed in MCF7 cells using PPP2R1B Polyclonal Antibody (Product # PA5-93159).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PPP2R1B in paraffin-embedded mouse lung using PPP2R1B Polyclonal Antibody (Product # PA5-93159) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PPP2R1B in paraffin-embedded mouse lung using PPP2R1B Polyclonal Antibody (Product # PA5-93159) at a dilution of 1:200.
