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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF567 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Neuropilin-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat Neuropilin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Neuropilin-2 and recombinant rat Neuropilin-1 is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program.
Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction.
Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis.
Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins.
Mechanotransduction activates canonical Wnt/β-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves.
Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
BIG-2 mediates olfactory axon convergence to target glomeruli.
Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface.
Frye M, Taddei A, Dierkes C, Martinez-Corral I, Fielden M, Ortsäter H, Kazenwadel J, Calado DP, Ostergaard P, Salminen M, He L, Harvey NL, Kiefer F, Mäkinen T
Nature communications 2018 Apr 17;9(1):1511
Nature communications 2018 Apr 17;9(1):1511
Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction.
Tatin F, Renaud-Gabardos E, Godet AC, Hantelys F, Pujol F, Morfoisse F, Calise D, Viars F, Valet P, Masri B, Prats AC, Garmy-Susini B
JCI insight 2017 Jun 15;2(12)
JCI insight 2017 Jun 15;2(12)
Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis.
Kunath J, Delaroque N, Szardenings M, Neundorf I, Straub RH
Life sciences 2017 Jan 1;168:47-53
Life sciences 2017 Jan 1;168:47-53
Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins.
Ulvmar MH, Martinez-Corral I, Stanczuk L, Mäkinen T
Genesis (New York, N.Y. : 2000) 2016 Jun;54(6):350-8
Genesis (New York, N.Y. : 2000) 2016 Jun;54(6):350-8
Mechanotransduction activates canonical Wnt/β-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves.
Cha B, Geng X, Mahamud MR, Fu J, Mukherjee A, Kim Y, Jho EH, Kim TH, Kahn ML, Xia L, Dixon JB, Chen H, Srinivasan RS
Genes & development 2016 Jun 15;30(12):1454-69
Genes & development 2016 Jun 15;30(12):1454-69
Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
Baluk P, Adams A, Phillips K, Feng J, Hong YK, Brown MB, McDonald DM
The American journal of pathology 2014 May;184(5):1577-92
The American journal of pathology 2014 May;184(5):1577-92
Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson TL, Miura N, Hong YK, Koopman P, Harvey NL, François M
Developmental biology 2014 Feb 1;386(1):25-33
Developmental biology 2014 Feb 1;386(1):25-33
Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
Demyanenko GP, Mohan V, Zhang X, Brennaman LH, Dharbal KE, Tran TS, Manis PB, Maness PF
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 20;34(34):11274-87
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 20;34(34):11274-87
Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
The Journal of clinical investigation 2010 May;120(5):1694-707
The Journal of clinical investigation 2010 May;120(5):1694-707
BIG-2 mediates olfactory axon convergence to target glomeruli.
Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
Neuron 2008 Mar 27;57(6):834-46
Neuron 2008 Mar 27;57(6):834-46
Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M
The Journal of clinical investigation 2008 Jun;118(6):2062-75
The Journal of clinical investigation 2008 Jun;118(6):2062-75
Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface.
Ito K, Kawasaki T, Takashima S, Matsuda I, Aiba A, Hirata T
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 23;28(17):4414-22
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 23;28(17):4414-22
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Neuropilin-2 by Western Blot. Western blot shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuropilin-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Neuropilin-2 by Simple WesternTM. Simple Western lane view shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin-2 at approximately 140 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neuropilin-2 in Rat Brain. Neuropilin-2 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
