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Western blot
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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-47274 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neuropilin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Neuropilin-2 is observed, and approximately 25% cross-reactivity with recombinant rat (rr) Neuropilin-2 is observed and less than 5% cross-reactivity with rrNeuropilin-1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Smad3 Regulates Neuropilin 2 Transcription by Binding to its 5' Untranslated Region.
Xie X, Urabe G, Marcho L, Williams C, Guo LW, Kent KC
Journal of the American Heart Association 2020 Apr 21;9(8):e015487
Journal of the American Heart Association 2020 Apr 21;9(8):e015487
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-human Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47274) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Neuropilin-2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Neuropilin 2 in HUVEC human umbilical vein endothelial cells. Samples were incubated in Neuropilin 2 polyclonal antibody (Product # PA5-47274) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Neuropilin‚2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Neuropilin 2 in 0.2 mg/mL lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line. Samples were incubated in Neuropilin 2 polyclonal antibody (Product # PA5-47274) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. A specific band was detected for Neuropilin‚2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Neuropilin 2 in HUVEC human umbilical vein endothelial cells. Samples were incubated in Neuropilin 2 polyclonal antibody (Product # PA5-47274) followed by Phycoerythrin-conjugated anti-Goat IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Neuropilin 2 (NRP2) is reduced in arteries of Smad3-haploinsufficient (vs wild-type) mice. Wire injury experiments were performed with Smad3 +/+ and Smad3 +/- mice as described in the Methods section. Immunohistochemistry was performed on cross-sections of injured arteries collected 28 days after wire injury. A , Representative immunostained cross-sections. Negative staining: no primary antibody. Scale bar: 50 mum. B , Quantification of immunohistochemistry analysis. Colorimetric intensity (per image field) on a total of 7 cross-sections from 4 animals was averaged to calculate mean+-SEM (n=4 mice). Statistics: unpaired Student t test; * P
