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- References [12]
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- Western blot [2]
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- Product number
- AF2215 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Neuropilin-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Neuropilin-2 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q7LBX6- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
Promotion of metastasis of thyroid cancer cells via NRP-2-mediated induction.
Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology.
Class 3 semaphorins induce F-actin reorganization in human dendritic cells: Role in cell migration.
Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells.
Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
Neuropilin-2 Is upregulated in lung cancer cells during TGF-β1-induced epithelial-mesenchymal transition.
Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis.
Martinez-Martin N, Marcandalli J, Huang CS, Arthur CP, Perotti M, Foglierini M, Ho H, Dosey AM, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C
Cell 2018 Aug 23;174(5):1158-1171.e19
Cell 2018 Aug 23;174(5):1158-1171.e19
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
Volk-Draper LD, Hall KL, Wilber AC, Ran S
PloS one 2017;12(6):e0179257
PloS one 2017;12(6):e0179257
Promotion of metastasis of thyroid cancer cells via NRP-2-mediated induction.
Tu DG, Chang WW, Jan MS, Tu CW, Lu YC, Tai CK
Oncology letters 2016 Nov;12(5):4224-4230
Oncology letters 2016 Nov;12(5):4224-4230
Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology.
Dutta S, Roy S, Polavaram NS, Stanton MJ, Zhang H, Bhola T, Hönscheid P, Donohue TM Jr, Band H, Batra SK, Muders MH, Datta K
Cancer research 2016 Jan 15;76(2):418-28
Cancer research 2016 Jan 15;76(2):418-28
Class 3 semaphorins induce F-actin reorganization in human dendritic cells: Role in cell migration.
Curreli S, Wong BS, Latinovic O, Konstantopoulos K, Stamatos NM
Journal of leukocyte biology 2016 Dec;100(6):1323-1334
Journal of leukocyte biology 2016 Dec;100(6):1323-1334
Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells.
Wang HB, Zhang H, Zhang JP, Li Y, Zhao B, Feng GK, Du Y, Xiong D, Zhong Q, Liu WL, Du H, Li MZ, Huang WL, Tsao SW, Hutt-Fletcher L, Zeng YX, Kieff E, Zeng MS
Nature communications 2015 Feb 11;6:6240
Nature communications 2015 Feb 11;6:6240
Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perrière N, Guillevic O, Couraud PO, Uzan G
PloS one 2014;9(1):e84179
PloS one 2014;9(1):e84179
Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.
Van't Hull EF, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye JF, Doucey MA
Oncoimmunology 2014;3:e29080
Oncoimmunology 2014;3:e29080
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
Hägerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams RH, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
The EMBO journal 2013 Mar 6;32(5):629-44
The EMBO journal 2013 Mar 6;32(5):629-44
Neuropilin-2 Is upregulated in lung cancer cells during TGF-β1-induced epithelial-mesenchymal transition.
Nasarre P, Gemmill RM, Potiron VA, Roche J, Lu X, Barón AE, Korch C, Garrett-Mayer E, Lagana A, Howe PH, Drabkin HA
Cancer research 2013 Dec 1;73(23):7111-21
Cancer research 2013 Dec 1;73(23):7111-21
Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
Takeuchi H, Inokuchi K, Aoki M, Suto F, Tsuboi A, Matsuda I, Suzuki M, Aiba A, Serizawa S, Yoshihara Y, Fujisawa H, Sakano H
Cell 2010 Jun 11;141(6):1056-67
Cell 2010 Jun 11;141(6):1056-67
Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis.
Fassold A, Falk W, Anders S, Hirsch T, Mirsky VM, Straub RH
Arthritis and rheumatism 2009 Oct;60(10):2892-901
Arthritis and rheumatism 2009 Oct;60(10):2892-901
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Neuropilin-2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Neuropilin-2 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin-2 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2215) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Neuropilin-2 in Human Pancreatic Cancer Tissue. Neuropilin-2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2215) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
