Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS5818 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- NDUFS1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 662-707 of Human NDUFS1.
- Description
- The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. Through use of chaotropic agents, complex I can be separated into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1-7. NDUFS1, a 75kD protein, is the largest subunit of complex I, and is thought to be the first of the Fe-S proteins to accept electrons from an NADH-flavoprotein reductase within the complex. NDUFS1 may even form part of the active site crevice where NADH is oxidized. NDUFS1 is also a critical caspase substrate in mitochondria, and caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of NDUFS1 pAb at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:PC12 cell lysate