17464-1-AP

antibody from Proteintech Group

Targeting: FASTKD2 KIAA0971

Provider product page for 17464-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [19]
Comments [0]
Validations
Western blot [1]

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Product number: 17464-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17464-1-AP, RRID:AB_2101119
Product name: FASTKD2 antibody
Antibody type: Polyclonal
Description: KD/KO validated FASTKD2 antibody (Cat. #17464-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

FASTKD2 protein structure - 17464-1-AP shown in red.

Submitted references Let's make it clear: systematic exploration of mitochondrial DNA- and RNA-protein complexes by complexome profiling.

Potter A, Cabrera-Orefice A, Spelbrink JN
Nucleic acids research 2023 Oct 27;51(19):10619-10641

mtFociCounter for automated single-cell mitochondrial nucleoid quantification and reproducible foci analysis.

Rey T, Tábara LC, Prudent J, Minczuk M
Nucleic acids research 2023 Nov 27;51(21):e107

Visualization of Mitochondrial RNA Granules in Cultured Cells Using 5-Bromouridine Labeling.

Xavier VJ, Martinou JC
Methods in molecular biology (Clifton, N.J.) 2021;2192:69-73

Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.

Choi A, Barrientos A
Methods in molecular biology (Clifton, N.J.) 2021;2192:211-226

A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids.

Nasonovs A, Garcia-Diaz M, Bogenhagen DF
PloS one 2021;16(3):e0249047

Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.

Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, Senatore A, Monks DA, Liu BH, Leary SC, Cheng HM
Cell reports 2021 Sep 14;36(11):109704

The FASTK family proteins fine-tune mitochondrial RNA processing.

Ohkubo A, Van Haute L, Rudler DL, Stentenbach M, Steiner FA, Rackham O, Minczuk M, Filipovska A, Martinou JC
PLoS genetics 2021 Nov;17(11):e1009873

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Rey T, Zaganelli S, Cuillery E, Vartholomaiou E, Croisier M, Martinou JC, Manley S
Nature cell biology 2020 Oct;22(10):1180-1186

Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.

Wei X, Du M, Li D, Wen S, Xie J, Li Y, Chen A, Zhang K, Xu P, Jia M, Wen C, Zhou H, Lyu J, Yang Y, Fang H
Human mutation 2020 May;41(5):961-972

FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.

Fang R, Zhang B, Lu X, Jin X, Liu T
Journal of cellular biochemistry 2020 Mar;121(3):2458-2466

Mitochondrial Alkbh1 localizes to mtRNA granules and its knockdown induces the mitochondrial UPR in humans and C. elegans.

Wagner A, Hofmeister O, Rolland SG, Maiser A, Aasumets K, Schmitt S, Schorpp K, Feuchtinger A, Hadian K, Schneider S, Zischka H, Leonhardt H, Conradt B, Gerhold JM, Wolf A
Journal of cell science 2019 Oct 1;132(19)

The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.

Zaganelli S, Rebelo-Guiomar P, Maundrell K, Rozanska A, Pierredon S, Powell CA, Jourdain AA, Hulo N, Lightowlers RN, Chrzanowska-Lightowlers ZM, Minczuk M, Martinou JC
The Journal of biological chemistry 2017 Mar 17;292(11):4519-4532

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.

Boehm E, Zaganelli S, Maundrell K, Jourdain AA, Thore S, Martinou JC
Nucleic acids research 2017 Jun 2;45(10):6135-6146

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Antonicka H, Choquet K, Lin ZY, Gingras AC, Kleinman CL, Shoubridge EA
EMBO reports 2017 Jan;18(1):28-38

Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression.

Boehm E, Zornoza M, Jourdain AA, Delmiro Magdalena A, García-Consuegra I, Torres Merino R, Orduña A, Martín MA, Martinou JC, De la Fuente MA, Simarro M
The Journal of biological chemistry 2016 Dec 9;291(50):25877-25887

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Arroyo JD, Jourdain AA, Calvo SE, Ballarano CA, Doench JG, Root DE, Mootha VK
Cell metabolism 2016 Dec 13;24(6):875-885

FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.

Popow J, Alleaume AM, Curk T, Schwarzl T, Sauer S, Hentze MW
RNA (New York, N.Y.) 2015 Nov;21(11):1873-84

A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function.

Jourdain AA, Koppen M, Rodley CD, Maundrell K, Gueguen N, Reynier P, Guaras AM, Enriquez JA, Anderson P, Simarro M, Martinou JC
Cell reports 2015 Feb 24;10(7):1110-21

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Antonicka H, Shoubridge EA
Cell reports 2015 Feb 17;10(6):920-932

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse brain tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP(FASTKD2 antibody) at dilution of 1:1000
Sample type: tissue