NBP2-16521
antibody from Novus Biologicals
Targeting: FOXO3
AF6q21, FKHRL1, FOXO2, FOXO3A
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP2-16521 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal FOXO3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Gene expression profiles between cystic and solid vestibular schwannoma indicate susceptible molecules and pathways in the cystic formation of vestibular schwannoma.
Functional regulatory mechanism of smooth muscle cell-restricted LMOD1 coronary artery disease locus.
Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.
The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats.
Yan S, Wang Q, Huo Z, Yang T, Yin X, Wang Z, Zhang Z, Wu H
Functional & integrative genomics 2019 Jul;19(4):673-684
Functional & integrative genomics 2019 Jul;19(4):673-684
Functional regulatory mechanism of smooth muscle cell-restricted LMOD1 coronary artery disease locus.
Nanda V, Wang T, Pjanic M, Liu B, Nguyen T, Matic LP, Hedin U, Koplev S, Ma L, Franzén O, Ruusalepp A, Schadt EE, Björkegren JLM, Montgomery SB, Snyder MP, Quertermous T, Leeper NJ, Miller CL
PLoS genetics 2018 Nov;14(11):e1007755
PLoS genetics 2018 Nov;14(11):e1007755
Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.
Lu H, Samanta D, Xiang L, Zhang H, Hu H, Chen I, Bullen JW, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9
The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats.
Sinanoglu O, Yener AN, Ekici S, Midi A, Aksungar FB
Urology 2012 Dec;80(6):1392.e1-6
Urology 2012 Dec;80(6):1392.e1-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NBP2-16521] - 30 ug of whole 293T cell lysate. Antibody at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NBP2-16521] - 30 ug of whole cell lysate. A: Jurkat. B: Raji. Antibody at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NBP2-16521] - Non-transfected (-) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - A. Control with 2 ug of preimmune rabbit IgG B. IP of FOXO3A protein by 2 ug of FOXO3A antibody at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Huh-7 xenograft, using FOXO3A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - C-term detects FOXO3A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - C-term detects FOXO3A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min