- GTX100277 - GeneTex FOXO3 antibody

Chang CC, Li HH, Chang YT, Ho YJ, Hsieh LJ, Chiu PY, Cheng YS, Lin CL, Lai TJ
CNS neuroscience & therapeutics 2018 Jan;24(1):47-57

Zhang Y, Aguilar OA, Storey KB
PeerJ 2016;4:e2317

Yang WB, Chen PH, Hsu T 1st, Fu TF, Su WC, Liaw H, Chang WC, Hung JJ
Oncotarget 2014 Feb 15;5(3):740-53

Tseng HL, Li CJ, Huang LH, Chen CY, Tsai CH, Lin CN, Hsu HY
Toxicology and applied pharmacology 2012 Oct 1;264(1):104-13

Sinanoglu O, Yener AN, Ekici S, Midi A, Aksungar FB
Urology 2012 Dec;80(6):1392.e1-6

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) C2C12 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41983

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: 293T 7.5% SDS PAGE GTX100277 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX100277 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: Jurkat B: Raji 7.5% SDS PAGE GTX100277 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) C2C12 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXO3A protein stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 £gg of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 £gg of FOXO3A antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A(GTX100277) antibody at 1:500 dilution.

GTX100277