R31609

antibody from NSJ Bioreagents

Targeting: FOXO3 AF6q21, FKHRL1, FOXO2, FOXO3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31609

Provider

NSJ Bioreagents

Product name

FOXO3A Antibody

Antibody type

Polyclonal

Description

This highly specific FOXO3A antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human and rat samples.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the FOXO3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of FOXO3A antibody and Lane 1: rat thymus; 2: (r) NRK; 3: (r) PC12; 4: human HEPG2; 5: (h) HeLa; 6: (h) K562; 7: (h) Jurkat. Predicted/observed molecular weight ~70 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of FOXO3A antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human 293T, 2) human MCF7, 3) human Jurkat, 4) human HeLa, 5) rat lung and 6) rat ovary tissue lysate with FOXO3A antibody. Expected molecular weight: 71-90 kDa depending on glycosylation level.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with FOXO3A antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: FOXO3A antibody testing of human intestinal cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: FOXO3A antibody testing of human lung cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human pancreatic ductal adenocarcinoma tissue with FOXO3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human pancreatic ductal adenocarcinoma tissue with FOXO3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colorectal adenocarcinoma tissue with FOXO3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MCF7 cells with FOXO3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FOXO3A antibody.