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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB16950 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16950, RRID:AB_10616175
- Product name
- FOXO3 (phospho S253) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of FOXO3.
- Storage
- Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Down-regulation of manganese-superoxide dismutase through phosphorylation of FOXO3a by Akt in explanted vascular smooth muscle cells from old rats.
Direct transcriptional regulation of Bim by FoxO3a mediates STI571-induced apoptosis in Bcr-Abl-expressing cells.
Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation.
Li M, Chiu JF, Mossman BT, Fukagawa NK
The Journal of biological chemistry 2006 Dec 29;281(52):40429-39
The Journal of biological chemistry 2006 Dec 29;281(52):40429-39
Direct transcriptional regulation of Bim by FoxO3a mediates STI571-induced apoptosis in Bcr-Abl-expressing cells.
Essafi A, Fernández de Mattos S, Hassen YA, Soeiro I, Mufti GJ, Thomas NS, Medema RH, Lam EW
Oncogene 2005 Mar 31;24(14):2317-29
Oncogene 2005 Mar 31;24(14):2317-29
Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation.
Seoane J, Le HV, Shen L, Anderson SA, Massagué J
Cell 2004 Apr 16;117(2):211-23
Cell 2004 Apr 16;117(2):211-23
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from NIH/3T3 cells treated with using Serum.Lane 1 : Using FOXO3 (phospho S253) polyclonal antibody (Cat # PAB16950).Lane 2 : Using the same antibody preincubated with synthesized peptide.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3 (phospho S253) polyclonal antibody (Cat # PAB16950).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
