66428-1-Ig

antibody from Proteintech Group

Targeting: FOXO3 AF6q21, FKHRL1, FOXO2, FOXO3A

Provider product page for 66428-1-Ig

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Product number: 66428-1-Ig - Provider product page
Provider: Proteintech Group
Product name: FOXO3A antibody
Antibody type: Monoclonal
Description: KD/KO validated FOXO3A antibody (Cat. #66428-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 1F12D11
Vial size: 20ul, 150ul

FOXO3 protein structure - 66428-1-Ig shown in red.

Submitted references Exercise preconditioning prevents immobilization-induced skeletal muscle atrophy by activating Prmt1-p38/ATF2-Sesn1 signaling axis in C57BL/6J mice.

Yang X, Zou Y, Wang H, Niu Y, Fu L
Sports medicine and health science 2026 Mar;8(2):197-209

AURKA Activates FOXO3a to Form a Positive Feedback Loop in the Proliferation and Migration of Keloid Fibroblasts.

Chu X, Sun J, Dai S, Liang Y, Qian X, Xu J, Zhang J
Advances in wound care 2026 Mar;15(3):168-180

Maternal vitamin D deficiency during pregnancy impaired offspring's spatial learning and memory ability: Insights into the role of the CaMKK2/AMPK/FoxO3a pathway.

Sun X, Qiao Y, Han Y, Hou Y, Wang J, Lu S, Wang J, Qiu F
The Journal of nutritional biochemistry 2026 Mar;149:110196

Melatonin Ameliorates decaBDE-Induced Autism-Relevant Behaviors Through Promoting SIRT1/SIRT3/FOXO3a-Dependent Mitochondrial Quality Control.

Gao L, Shen J, Gao J, Li T, Yan D, Zeng X, Meng J, Li H, Chen D, Wu J
Antioxidants (Basel, Switzerland) 2026 Mar 23;15(3)

Polyphyllin VI induces Forkhead box O3 nuclear liquid-liquid phase separation to promote mitophagy in breast cancer.

Zhan X, Zhong Y, Liu X, Peng W, Zhang X, Xiang K, Yu Q, Chai Y, Chen Y, Liu Y, Si Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2026 Jun;155:158166

Tibetan medicine Bawei Chenxiang Wan attenuates chronic mountain sickness by targeting the AKT/FOXO3a/CAT axis to inhibit oxidative stress.

Li X, Zhang S, Qiu Y, Wang Y, Hou J, Ji X, Ge F, Zhang X, Sun Q, Song H, Ciwang R, Luo Y, Tao X
Journal of ethnopharmacology 2026 Jun 12;364:121481

Tumor-Derived Prostaglandin E2 Promotes Colorectal Cancer Progression by Inducing N2-Type TAN Polarization.

Han Y, Chen W, Zhang B, Wang S
Cancer science 2026 Jan;117(1):19-32

Mitochondrial NAD(+)-mediated mitophagy alleviates type I interferon response to the cytosolic mitochondrial DNA.

Lan T, Shang D, Lin L, Wang H, Zou J, Hu M, Cheng H, Zhou R
Autophagy 2026 Feb;22(2):266-284

FoxO3a regulated by miR-150-5p promotes the pyroptosis of macrophages in atherosclerosis.

Cai PY, Li MM, Chen SH, Zheng YL, Li J, Huang DH, Du JR, Lin HL
PloS one 2025;20(10):e0327075

Single-cell RNA sequencing reveals prolonged polyacrylamide hydrogel stimulation in vivo leads to an immunosuppressive microenvironment and potential tumorigenesis.

Liu Z, Sun M, Lu L, Wu X, Wang H, Gao Y, Liu A, Qian Y, Hu H, Jiang H
Computers in biology and medicine 2025 Sep;196(Pt B):110828

FOXO3a-BAP1 axis regulates neuronal ferroptosis in early brain injury after subarachnoid hemorrhage.

Liu C, Tian Q, Li Z, Wang G, Han W, Jiang S, Sun Z, Xu Q, Wang L, Liao J, Li M
Redox biology 2025 May;82:103550

Effective compound combination of Bufei Yishen formula ameliorates PM2.5-induced COPD by inhibiting mitochondrial oxidative stress through SIRT3-mediated FOXO3 deacetylation.

Wang J, Yue H, Dong Y, Liu T, Li J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 May;140:156568

Glutamine transporter inhibitor enhances the sensitivity of NSCLC to trametinib through GSDME-dependent pyroptosis.

Liu Q, Hu J, Li X, Gao H, Kong D, Jin M
Biochemical pharmacology 2025 Mar;233:116796

USP25 ameliorates vascular remodeling by deubiquitinating FOXO3 and promoting autophagic degradation of FOXO3.

Chen Y, Ye B, Xu D, Lin W, Fang Z, Qu X, Han X, Luo W, Chen C, Huang W, Zhou H, Wu G, Wang Y, Liang G
Acta pharmaceutica Sinica. B 2025 Mar;15(3):1643-1658

Aerobic plus resistance exercise attenuates skeletal muscle atrophy induced by dexamethasone through the HDAC4/FoxO3a pathway.

Liang D, Wang D, Zheng X, Xiang H, Liu S, Yu C, Tian J, Ma J, Niu Y
Cellular signalling 2025 Mar;127:111581

Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus.

Xian Y, Liu B, Shen T, Yang L, Peng R, Shen H, An X, Wang Y, Ben Y, Jiang Q, Guo B
Bone research 2025 Mar 3;13(1):30

Integrated transcriptomics and metabolomics studies reveal the therapeutic effects of Astragalus polysaccharides on cancer cachexia muscle atrophy.

Tian Z, Wang Y, Liu X, Xin X, Liu S, Qu Y, Li Z, Su X, Ma D
Scientific reports 2025 Jul 19;15(1):26224

Blocking of SIRT7/FOXO3a axis by miR-152-3p enhances cisplatin sensitivity in breast cancer.

Shi X, Ji Y, Wu X, Du Y, Yan X, Wang Y, Xia X
The American journal of the medical sciences 2025 Jan;369(1):105-115

Mammalian Sterile 20-Like Kinase 2 Knockdown Alleviates Neuropathic Pain in Rats by Mitochondrial Protection.

Zeng Q, Huang BX, Liu C, Liang N, Yu J, Liao SJ
Molecular neurobiology 2025 Dec;62(12):15628-15646

Sirtuin 3 promotes osteogenic differentiation of bone marrow mesenchymal stem cells by regulating macrophage polarization under high glucose.

Lin L, Ren Y, Wang X, Liang H, Yao Q
Scientific reports 2025 Dec 25;16(1):3515

Curcumin preserves bone health compromised by diabetes by inhibiting osteoporosis through regulation of the SIRT3/FoxO3a signalling pathway.

Mohammad OH, Yang S, Ji W, Ma H, Tao R
Scientific reports 2025 Aug 12;15(1):29566

Extracellular vesicles from neural stem cells safeguard neurons in intracerebral hemorrhage by suppressing reactive astrocyte neurotoxicity.

Li T, Zhang L, Wang P, Yu J, Zhong J, Tang Q, Zhu T, Chen K, Li F, Hong P, Wei J, Sun X, Ji G, Song B, Zhu J
Cell reports 2024 Oct 22;43(10):114854

SPIN1 accelerates tumorigenesis and confers radioresistance in non-small cell lung cancer by orchestrating the FOXO3a/FOXM1 axis.

Zhong M, Fang Z, Zou J, Chen X, Qiu Z, Zhou L, Le Y, Chen Z, Liao Y, Nie F, Wei X, Zhan J, Xiong J, Xiang X, Fang Z
Cell death & disease 2024 Nov 15;15(11):832

N-Acetylneuraminic acid triggers endothelial pyroptosis and promotes atherosclerosis progression via GLS2-mediated glutaminolysis pathway.

Liu Z, Xiang P, Zeng S, Weng P, Wen Y, Zhang W, Hu H, Zhao D, Ma L, Yu C
Cell death discovery 2024 Nov 13;10(1):467

CDK12 inhibition upregulates ATG7 triggering autophagy via AKT/FOXO3 pathway and enhances anti-PD-1 efficacy in colorectal cancer.

Wu Z, Zhang W, Chen L, Wang T, Wang X, Shi H, Zhang L, Zhong M, Shi X, Mao X, Chen H, Li Q
Pharmacological research 2024 Mar;201:107097

BNC1 deficiency induces mitochondrial dysfunction-triggered spermatogonia apoptosis through the CREB/SIRT1/FOXO3 pathway: the therapeutic potential of nicotinamide riboside and metformin†.

Ni F, Wang F, Li J, Liu Y, Sun X, Chen J, Li J, Zhang Y, Jin J, Ye X, Tu M, Chen J, Chen C, Zhang D
Biology of reproduction 2024 Mar 13;110(3):615-631

Tobacco toxins trigger bone marrow mesenchymal stem cells aging by inhibiting mitophagy.

Xiang K, Ren M, Liu F, Li Y, He P, Gong X, Chen T, Wu T, Huang Z, She H, Liu K, Jing Z, Yang S
Ecotoxicology and environmental safety 2024 Jun 1;277:116392

Adiponectin inhibits ROS/NLRP3 inflammatory pathway through FOXO3A to ameliorate oral submucosal fibrosis.

Zeng Y, Luo M, Yao Z, Xiao X
Odontology 2024 Jul;112(3):811-825

NADase CD38 is a key determinant of ovarian aging.

Yang Q, Chen W, Cong L, Wang M, Li H, Wang H, Luo X, Zhu J, Zeng X, Zhu Z, Xu Y, Lei M, Zhao Y, Wei C, Sun Y
Nature aging 2024 Jan;4(1):110-128

Microglial FoxO3a deficiency ameliorates ferroptosis-induced brain injury of intracerebral haemorrhage via regulating autophagy and heme oxygenase-1.

Wang R, Liang Z, Xue X, Mei H, Ji L, Wang B, Chen W, Gao C, Yuan S, Wu T, Qi H, Hu S, Yi L, Song Y, Liao R, Chen B
Journal of cellular and molecular medicine 2024 Jan;28(1):e18007

Gastrodin alleviates cisplatin nephrotoxicity by inhibiting ferroptosis via the SIRT1/FOXO3A/GPX4 signaling pathway.

Qiu CW, Chen B, Zhu HF, Liang YL, Mao LS
Journal of ethnopharmacology 2024 Jan 30;319(Pt 3):117282

Copper overload induces apoptosis and impaired proliferation of T cell in zebrafish.

Li L, Shi J, Liu W, Luo Y, Gao S, Liu JX
Aquatic toxicology (Amsterdam, Netherlands) 2024 Feb;267:106808

Integration of single-nucleus and exosome RNA sequencing dissected inter-cellular communication and biomarkers in pancreatic ductal adenocarcinoma.

Tang R, Zhang Z, Xu J, Wang W, Meng Q, Liu Y, Du Q, Liang C, Hua J, Zhang B, Yu X, Shi S
Computational and structural biotechnology journal 2024 Dec;23:1689-1704

TRIM6 promotes glioma malignant progression by enhancing FOXO3A ubiquitination and degradation.

Guo J, Wang J, Zhang P, Wen P, Zhang S, Dong X, Dong J
Translational oncology 2024 Aug;46:101999

EEPD1 attenuates radiation-induced cardiac hypertrophy and apoptosis by degrading FOXO3A in cardiomyocytes.

Yu K, Su X, Zhou T, Cai X, Zhang M
Acta biochimica et biophysica Sinica 2024 Aug 29;56(12):1733-1747

Galaxamide alleviates cisplatin-induced premature ovarian insufficiency via the PI3K signaling pathway in HeLa tumor-bearing mice.

Tao H, Chen Z, Yao B, Ren X, Shuai H, Xu S, Zha Q, Li P
BMC cancer 2024 Aug 28;24(1):1060

Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease.

Guo Q, Wang Y, Yu L, Guan L, Ji X, Li X, Pang G, Ren Z, Ye L, Cheng H
Journal of translational medicine 2024 Apr 12;22(1):350

Grape seed proanthocyanidins regulate mitophagy of endothelial cells and promote wound healing in mice through p-JNK/FOXO3a/ROS signal pathway.

Chen L, Hao L, Yanshuo C, FangFang W, Daqin C, Weidong X, Jian X, Shaodong C, Hongyu Z, Ke X
Archives of biochemistry and biophysics 2023 Nov;749:109790

Cellular Localization of FOXO3 Determines Its Role in Cataractogenesis.

Li X, Qu Y, Yang Q, Li R, Diao Y, Wang J, Wu L, Zhang C, Cui S, Qin L, Zhuo D, Wang H, Wang L, Huang Y
The American journal of pathology 2023 Nov;193(11):1845-1862

The metabolite butyrate produced by gut microbiota inhibits cachexia-associated skeletal muscle atrophy by regulating intestinal barrier function and macrophage polarization.

Liu H, Xi Q, Tan S, Qu Y, Meng Q, Zhang Y, Cheng Y, Wu G
International immunopharmacology 2023 Nov;124(Pt B):111001

Tumor-Derived Oxidative Stress Triggers Ovarian Follicle Loss in Breast Cancer.

Wu Y, Huang J, Chen H, Tao H, He Y, Yang G, Zha Q, Lash GE, Li P
The American journal of pathology 2023 May;193(5):608-623

PARM1 Drives Smooth Muscle Cell Proliferation in Pulmonary Arterial Hypertension via AKT/FOXO3A Axis.

He Z, Chang T, Chen Y, Wang H, Dai L, Zeng H
International journal of molecular sciences 2023 Mar 28;24(7)

The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcoma.

Niu J, Yan T, Guo W, Wang W, Ren T, Huang Y, Zhao Z, Yu Y, Chen C, Huang Q, Lou J, Guo L
Autophagy 2023 Jun;19(6):1693-1710

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Zhong S, Chen W, Wang B, Gao C, Liu X, Song Y, Qi H, Liu H, Wu T, Wang R, Chen B
Redox biology 2023 Jul;63:102760

Ginsenoside Rh2 inhibits CBP/p300-mediated FOXO3a acetylation and epilepsy-induced oxidative damage via the FOXO3a-KEAP1-NRF2 pathway.

Wu J, Wang S, Zhao W, Li M, Li S
European journal of pharmacology 2023 Feb 5;940:175391

METTL14-m6A-FOXO3a axis regulates autophagy and inflammation in ankylosing spondylitis.

Chen Y, Wu Y, Fang L, Zhao H, Xu S, Shuai Z, Yu H, Cai G, Zhan HQ, Pan F
Clinical immunology (Orlando, Fla.) 2023 Dec;257:109838

Role of omentin-1 in susceptibility to anxiety and depression like behaviors.

Ji L, Zhang L, Liang Z, Zhong S, Liu X, Liu Z, Poon WS, Song Y, Chen B, Wang R
Molecular and cellular endocrinology 2023 Aug 20;574:111990

Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis.

Zhao CQ, Wang C, Liu MM, Cao M, Peng J, Kong DQ, Ren XT, Liu R, Hai CX, Zhang XD
Scientific reports 2023 Apr 26;13(1):6833

Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC.

Du X, Zhang X, Dong J, Zou N, Guo D, Yao W, Wang X, Li S, Song C, Yan K, Shen W, Zhu S
Journal of translational medicine 2022 Nov 5;20(1):507

Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway.

Zhang P, Tian L, Bao J, Li S, Li A, Wen Y, Wang J, Jie Y
Investigative ophthalmology & visual science 2022 Mar 2;63(3):29

Estradiol initiates the immune escape of non-small cell lung cancer cells via ERβ/SIRT1/FOXO3a/PD-L1 axis.

Song S, Tang H, Quan W, Shang A, Ling C
International immunopharmacology 2022 Jun;107:108629

Icaritin inhibits PLK1 to activate DNA damage response in NK/T cell lymphoma and increases sensitivity to GELOX regime.

Zhang C, Xu H, Sui X, Chen L, Chen B, Lv H, Wang S, Wang X
Molecular therapy oncolytics 2022 Jun 16;25:288-304

Honokiol improves cognitive impairment in APP/PS1 mice through activating mitophagy and mitochondrial unfolded protein response.

Hou M, Bao W, Gao Y, Chen J, Song G
Chemico-biological interactions 2022 Jan 5;351:109741

FOXO3 Mediates Tooth Movement by Regulating Force-Induced Osteogenesis.

Jin A, Hong Y, Yang Y, Xu H, Huang X, Gao X, Gong X, Dai Q, Jiang L
Journal of dental research 2022 Feb;101(2):196-205

CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinoma.

Yao J, Wang J, Xu Y, Guo Q, Sun Y, Liu J, Li S, Guo Y, Wei L
Autophagy 2022 Aug;18(8):1879-1897

Inhibition of the cardiac fibroblast-enriched histone methyltransferase Dot1L prevents cardiac fibrosis and cardiac dysfunction.

Xu J, Wang J, Long F, Zhong W, Su H, Su Z, Liu X
Cell & bioscience 2022 Aug 19;12(1):134

Advanced oxidation protein products upregulate ABCB1 expression and activity via HDAC2-Foxo3α-mediated signaling in vitro and in vivo.

Xun T, Lin Z, Zhang M, Mo L, Chen Y, Wang X, Zhao J, Ye C, Feng H, Yang X
Toxicology and applied pharmacology 2022 Aug 15;449:116140

ATIC inhibits autophagy in hepatocellular cancer through the AKT/FOXO3 pathway and serves as a prognostic signature for modeling patient survival.

Zhang H, Xia P, Liu J, Chen Z, Ma W, Yuan Y
International journal of biological sciences 2021;17(15):4442-4458

FOXO3A-induced LINC00926 suppresses breast tumor growth and metastasis through inhibition of PGK1-mediated Warburg effect.

Chu Z, Huo N, Zhu X, Liu H, Cong R, Ma L, Kang X, Xue C, Li J, Li Q, You H, Zhang Q, Xu X
Molecular therapy : the journal of the American Society of Gene Therapy 2021 Sep 1;29(9):2737-2753

Psychosocial and behavioral problems of children and adolescents in the early stage of reopening schools after the COVID-19 pandemic: a national cross-sectional study in China.

Wang L, Zhang Y, Chen L, Wang J, Jia F, Li F, Froehlich TE, Hou Y, Hao Y, Shi Y, Deng H, Zhang J, Huang L, Xie X, Fang S, Xu L, Xu Q, Guan H, Wang W, Shen J, Qian Y, Wang X, Shan L, Tan C, Yu Y, Wang X, Chen F, Zhang L, Li X, Shi X, Ke X, Li T
Translational psychiatry 2021 Jun 3;11(1):342

Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway.

Tan J, Luo J, Meng C, Jiang N, Cao J, Zhao J
International immunopharmacology 2021 Jan;90:107268

SIRT2 tyrosine nitration by peroxynitrite in response to renal ischemia/reperfusion injury.

Wang Y, Wu CJ, Du Y, Liu YQ, Cai JR, Wu XQ, Hu SQ
Free radical research 2021 Dec;55(11-12):1104-1118

Protective effect of limonin against doxorubicin-induced cardiotoxicity via activating nuclear factor - like 2 and Sirtuin 2 signaling pathways.

Deng J, Huang M, Wu H
Bioengineered 2021 Dec;12(1):7975-7984

Alleviation of CCCP-induced mitochondrial injury by augmenter of liver regeneration via the PINK1/Parkin pathway-dependent mitophagy.

Zhang J, Chen S, Li Y, Xiao W, An W
Experimental cell research 2021 Dec 1;409(1):112866

Association of T2285C polymorphism in PARP1 gene coding region with its expression, activity and NSCLC risk along with prognosis.

Wang Y, Xia XB, Tang HZ, Cai JR, Shi XK, Ji HX, Yan XN, Xu T
Mutagenesis 2021 Aug 27;36(4):281-293

Solasonine Suppresses the Proliferation of Acute Monocytic Leukemia Through the Activation of the AMPK/FOXO3A Axis.

Zhang H, Tian F, Jiang P, Qian S, Dai X, Ma B, Wang M, Dai H, Sha X, Yang Z, Zhu X, Sun X
Frontiers in oncology 2020;10:614067

Altered m(6) A modification is involved in up-regulated expression of FOXO3 in luteinized granulosa cells of non-obese polycystic ovary syndrome patients.

Zhang S, Deng W, Liu Q, Wang P, Yang W, Ni W
Journal of cellular and molecular medicine 2020 Oct;24(20):11874-11882

Next generation miRNA inhibition using short anti-seed PNAs encapsulated in PLGA nanoparticles.

Malik S, Lim J, Slack FJ, Braddock DT, Bahal R
Journal of controlled release : official journal of the Controlled Release Society 2020 Nov 10;327:406-419

TGR5 deficiency aggravates hepatic ischemic/reperfusion injury via inhibiting SIRT3/FOXO3/HIF-1ɑ pathway.

Wang Q, Wei S, Li L, Qiu J, Zhou S, Shi C, Shi Y, Zhou H, Lu L
Cell death discovery 2020 Nov 1;6(1):116

Role of FOXO3 Activated by HIV-1 Tat in HIV-Associated Neurocognitive Disorder Neuronal Apoptosis.

Dong H, Ye X, Zhong L, Xu J, Qiu J, Wang J, Shao Y, Xing H
Frontiers in neuroscience 2019;13:44

Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3α Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs.

Yang G, Jin L, Zheng D, Tang X, Yang J, Fan L, Xie X
Marine drugs 2019 Dec 12;17(12)

Protective effects of 2,3,5,4-tetrahydroxystilbene-2-o-β-D-glucoside against osteoporosis: Current knowledge and proposed mechanisms.

Zhang J, Li S, Wei L, Peng Y, Zheng Z, Xue J, Cao Y, Wang B, Du J
International journal of rheumatic diseases 2018 Aug;21(8):1504-1513

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