Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-20973 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXO3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is A-20 cell lysate. PA5-20973 can be used with blocking peptide PEP-1087.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Atrogin-1/MAFbx mRNA expression is regulated by histone deacetylase 1 in rat soleus muscle under hindlimb unloading.
Mochalova EP, Belova SP, Mirzoev TM, Shenkman BS, Nemirovskaya TL
Scientific reports 2019 Jul 16;9(1):10263
Scientific reports 2019 Jul 16;9(1):10263
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A-20 cell lysate using a FOXO3 polyclonal antibody (Product # PA5-20973) at (A) 0.5 and (B) 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FOXO3 in A-20 cell lysate with FOXO3A Polyclonal Antibody (Product # PA5-20973) at (A) 0.5 and (B) 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of A-20 cells using a FOXO3 polyclonal antibody (Product # PA5-20973) at a 4 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of FOXO3 in A-20 cells with FOXO3A Polyclonal Antibody (Product # PA5-20973) at 4 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 The content of phosphorylated Akt (S473) (A) and FoxO3 (S253) (B) in rat soleus muscle. S -control, HS - 3-day hindlimb suspension + placebo, HS + CI - 3-day hindlimb suspension + CI-994 (HDAC1 inhibitor). All values are means +- SE, n = 8/group. *significant difference from control (p < 0.05).