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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [8]
- Immunohistochemistry [8]
- Other assay [1]
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- Product number
- PA5-28206 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HepG2, HeLa, Huh-7, Neuro2A , C8D30 , NIH-3T3 , Raw 264.7 , C2Cl2, DDDDk-tagged IDH1-transfected 293T. Predicted reactivity: Mouse (96%), Rat (97%), Zebrafish (85%), Xenopus laevis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Single-Cell Transcriptomic Atlas of Primate Ovarian Aging.
Wang S, Zheng Y, Li J, Yu Y, Zhang W, Song M, Liu Z, Min Z, Hu H, Jing Y, He X, Sun L, Ma L, Esteban CR, Chan P, Qiao J, Zhou Q, Izpisua Belmonte JC, Qu J, Tang F, Liu GH
Cell 2020 Feb 6;180(3):585-600.e19
Cell 2020 Feb 6;180(3):585-600.e19
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IDH1 using 30 µg of A) A431 and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IDH1 polyclonal antibody (Product # PA5-28206) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IDH1 using 30 µg of A) JC and B) C2C12 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IDH1 polyclonal antibody (Product # PA5-28206) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDH1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IDH1 Polyclonal Antibody (Product # PA5-28206) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDH1 was performed by separating 30 µg of various whole cell lysates/extracts by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a IDH1 Polyclonal Antibody (Product # PA5-28206) at a dilution of 1:1000. A. Neuro2A, B. C8D30, C. NIH-3T3, D. Raw 264.7, E. C2Cl2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IDH1 Polyclonal Antibody (Product # PA5-28206). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 Polyclonal Antibody (Product # PA5-28206) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDH1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IDH1 Polyclonal Antibody (Product # PA5-28206) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDH1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IDH1 Polyclonal Antibody (Product # PA5-28206) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDH1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a IDH1 Polyclonal Antibody (Product # PA5-28206) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of IDH1 was performed in paraffin-embedded rat colon tissue using IDH1 Polyclonal Antibody (Product # PA5-28206) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of IDH1 was performed in paraffin-embedded mouse duodenum tissue using IDH1 Polyclonal Antibody (Product # PA5-28206) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDH1 Polyclonal Antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. IDH1 stained by IDH1 Polyclonal Antibody (Product # PA5-28206) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDH1 Polyclonal Antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. IDH1 stained by IDH1 Polyclonal Antibody (Product # PA5-28206) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDH1 Polyclonal Antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. IDH1 stained by IDH1 Polyclonal Antibody (Product # PA5-28206) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDH1 Polyclonal Antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. IDH1 stained by IDH1 Polyclonal Antibody (Product # PA5-28206) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDH1 Polyclonal Antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. IDH1 stained by IDH1 Polyclonal Antibody (Product # PA5-28206) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IDH1 (Product # PA5-28206) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 µg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 µg of IDH-1 antibody (Product # PA5-28206) 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody (Product # PA5-28206) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
