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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX101025 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101025, RRID:AB_1241223
- Product name
- PTEN antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Oxymatrine induces apoptosis and inhibits invasion in Gallbladder carcinoma via PTEN/PI3K/AKT pathway.
miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells.
MicroRNA-200c Promotes Suppressive Potential of Myeloid-Derived Suppressor Cells by Modulating PTEN and FOG2 Expression.
Prostate tumorigenesis induced by PTEN deletion involves estrogen receptor β repression.
RNA-seq reveals aurora kinase-driven mTOR pathway activation in patients with sarcomatoid metastatic renal cell carcinoma.
HOXA10 is associated with temozolomide resistance through regulation of the homologous recombinant DNA repair pathway in glioblastoma cell lines.
HOXA10 is associated with temozolomide resistance through regulation of the homologous recombinant DNA repair pathway in glioblastoma cell lines.
UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.
Qian L, Li X, Ye P, Wang G, Dai W, Liu Y, Gao Q, Shen G
Cytotechnology 2018 Feb;70(1):83-94
Cytotechnology 2018 Feb;70(1):83-94
miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Liu J, Chen W, Zhang H, Liu T, Zhao L
Oncology letters 2017 Nov;14(5):5711-5718
Oncology letters 2017 Nov;14(5):5711-5718
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A
International journal of molecular sciences 2017 Dec 4;18(12)
International journal of molecular sciences 2017 Dec 4;18(12)
Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells.
Chen NC, Chyau CC, Lee YJ, Tseng HC, Chou FP
Scientific reports 2016 Feb 3;6:20417
Scientific reports 2016 Feb 3;6:20417
MicroRNA-200c Promotes Suppressive Potential of Myeloid-Derived Suppressor Cells by Modulating PTEN and FOG2 Expression.
Mei S, Xin J, Liu Y, Zhang Y, Liang X, Su X, Yan H, Huang Y, Yang R
PloS one 2015;10(8):e0135867
PloS one 2015;10(8):e0135867
Prostate tumorigenesis induced by PTEN deletion involves estrogen receptor β repression.
Mak P, Li J, Samanta S, Chang C, Jerry DJ, Davis RJ, Leav I, Mercurio AM
Cell reports 2015 Mar 31;10(12):1982-91
Cell reports 2015 Mar 31;10(12):1982-91
RNA-seq reveals aurora kinase-driven mTOR pathway activation in patients with sarcomatoid metastatic renal cell carcinoma.
Pal SK, He M, Tong T, Wu H, Liu X, Lau C, Wang JH, Warden C, Wu X, Signoretti S, Choueiri TK, Karam JA, Jones JO
Molecular cancer research : MCR 2015 Jan;13(1):130-7
Molecular cancer research : MCR 2015 Jan;13(1):130-7
HOXA10 is associated with temozolomide resistance through regulation of the homologous recombinant DNA repair pathway in glioblastoma cell lines.
Kim JW, Kim JY, Kim JE, Kim SK, Chung HT, Park CK
Genes & cancer 2014 May;5(5-6):165-74
Genes & cancer 2014 May;5(5-6):165-74
HOXA10 is associated with temozolomide resistance through regulation of the homologous recombinant DNA repair pathway in glioblastoma cell lines.
Kim JW, Kim JY, Kim JE, Kim SK, Chung HT, Park CK
Genes & cancer 2014 May;5(5-6):165-174
Genes & cancer 2014 May;5(5-6):165-174
UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.
Huang AM, Kao YT, Toh S, Lin PY, Chou CH, Hu HT, Lu CY, Liou JY, Chao SY, Hour TC, Pu YS
Biochemical pharmacology 2011 Jul 1;82(1):35-42
Biochemical pharmacology 2011 Jul 1;82(1):35-42
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX101025 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX101025 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE GTX101025 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PTEN antibody detects PTEN protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTEN(GTX101025) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PTEN antibody detects PTEN protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PTEN antibody (GTX101025) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PTEN antibody detects PTEN protein at cytosol and nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. PTEN antibody (GTX101025) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PTEN antibody detects PTEN protein at cytoplasm in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. PTEN antibody (GTX101025) diluted at 1:500.
