F51131

antibody from NSJ Bioreagents

Targeting: PTEN BZS, MHAM, MMAC1, PTEN1, TEP1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F51131

Provider

NSJ Bioreagents

Product name

PTEN Antibody

Antibody type

Polyclonal

Description

This highly specific PTEN antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the PTEN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of PTEN antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTEN gene (2).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

PTEN antibody western blot analysis in HL-60 lysate

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of PTEN antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human lung carcinoma tissue stained with PTEN antibody