APRab16639

antibody from EnkiLife Biotech Co., Ltd.

Targeting: PTEN BZS, MHAM, MMAC1, PTEN1, TEP1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

APRab16639

Provider

EnkiLife Biotech Co., Ltd.

Product name

PTEN Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Affinity purification

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Polyclonal

Vial size

100 µl

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Western Blot analysis of mouse-heart mouse-brain mouse-lung Hela KB SH-SY5Y cells using PTEN Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunocytochemistry

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of human-heart tissue. 1,PTEN Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of human-heart tissue. 1,PTEN Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of human-lung tissue. 1,PTEN Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of human-lung tissue. 1,PTEN Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of rat-spleen tissue. 1,PTEN Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of rat-spleen tissue. 1,PTEN Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemistry

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200