PA5-79355

antibody from Invitrogen Antibodies

Targeting: HDGF HMG1L2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79355

Provider

Invitrogen Antibodies

Product name

HDGF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human A549 whole cell, human 293T whole cell, human Hela whole cell, rat C6 whole cell. IHC: mouse liver tissue, rat liver tissue, human intestinal cancer tissue, human lung cancer tissue. ICC/IF: U20S cell. Flow: A431 cell.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HDGF in, Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat C6 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with HDGF Polyclonal Antibody (Product # PA5-79355) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for HDGF at approximately 38 kDa. The expected band size for HDGF is at 27 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HDGF using anti-HDGF antibody (Product # PA5-79355) . HDGF was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-HDGF antibody (Product # PA5-79355) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HDGF using anti-HDGF antibody (Product # PA5-79355) . HDGF was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-HDGF antibody (Product # PA5-79355) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDGF on paraffin-embedded human lung cancer tissue. Sample was incubated with HDGF polyclonal antibody (Product# PA5-79355) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDGF on paraffin-embedded rat liver tissue. Sample was incubated with HDGF polyclonal antibody (Product# PA5-79355) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDGF on paraffin-embedded human intestinal cancer tissue. Sample was incubated with HDGF polyclonal antibody (Product# PA5-79355) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDGF on paraffin-embedded mouse liver tissue. Sample was incubated with HDGF polyclonal antibody (Product# PA5-79355) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDGF on paraffin-embedded rat liver tissue. Sample was incubated with HDGF polyclonal antibody (Product# PA5-79355) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) method.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of HDGF in A431 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with HDGF Polyclonal Antibody (Product # PA5-79355) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of HDGF in A431 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with HDGF Polyclonal Antibody (Product # PA5-79355) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.