Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-3079-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD307d (FcRL4) Monoclonal Antibody (413D12), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 413D12 monoclonal antibody reacts with human CD307d, which is also known as FcRL4, Fc receptor-like 4, FcRH4, IRTA4, or IGFP2. CD307d is an inhibitory receptor that is highly expressed on a subset of memory B cells resident in epithelial tissues and marginal zones of mucosal lymphoid tissues such as tonsils. CD307d has been shown to recruit the phosphatase SHP-1, thereby acting as an inhibitory receptor in B cells. CD307d is homologous to FcgammaRI and specifically binds IgA. To date, CD307d is the only known inhibitory receptor for IgA. Expression of CD307d is increased on memory B cells from patients with chronic infections such as HIV, suggesting that enhanced expression of inhibitory receptors, such as CD307d, may contribute to the inefficiency of antibody responses against HIV. Applications Reported: This 413D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 413D12 antibody has been pre-titrated and tested by flow cytometric analysis of human tonsil tissue or transfected 293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 413D12
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG.
Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
FcRL4 acts as an adaptive to innate molecular switch dampening BCR signaling and enhancing TLR signaling.
Discriminating gene expression profiles of memory B cell subpopulations.
Expression of the immunoregulatory molecule FcRH4 defines a distinctive tissue-based population of memory B cells.
IRTA1 and IRTA2, novel immunoglobulin superfamily receptors expressed in B cells and involved in chromosome 1q21 abnormalities in B cell malignancy.
Wilson TJ, Fuchs A, Colonna M
Journal of immunology (Baltimore, Md. : 1950) 2012 May 15;188(10):4741-5
Journal of immunology (Baltimore, Md. : 1950) 2012 May 15;188(10):4741-5
Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
Llinàs L, Lázaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
Immunology letters 2011 Jan 30;134(2):113-21
Immunology letters 2011 Jan 30;134(2):113-21
FcRL4 acts as an adaptive to innate molecular switch dampening BCR signaling and enhancing TLR signaling.
Sohn HW, Krueger PD, Davis RS, Pierce SK
Blood 2011 Dec 8;118(24):6332-41
Blood 2011 Dec 8;118(24):6332-41
Discriminating gene expression profiles of memory B cell subpopulations.
Ehrhardt GR, Hijikata A, Kitamura H, Ohara O, Wang JY, Cooper MD
The Journal of experimental medicine 2008 Aug 4;205(8):1807-17
The Journal of experimental medicine 2008 Aug 4;205(8):1807-17
Expression of the immunoregulatory molecule FcRH4 defines a distinctive tissue-based population of memory B cells.
Ehrhardt GR, Hsu JT, Gartland L, Leu CM, Zhang S, Davis RS, Cooper MD
The Journal of experimental medicine 2005 Sep 19;202(6):783-91
The Journal of experimental medicine 2005 Sep 19;202(6):783-91
IRTA1 and IRTA2, novel immunoglobulin superfamily receptors expressed in B cells and involved in chromosome 1q21 abnormalities in B cell malignancy.
Hatzivassiliou G, Miller I, Takizawa J, Palanisamy N, Rao PH, Iida S, Tagawa S, Taniwaki M, Russo J, Neri A, Cattoretti G, Clynes R, Mendelsohn C, Chaganti RS, Dalla-Favera R
Immunity 2001 Mar;14(3):277-89
Immunity 2001 Mar;14(3):277-89
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells (left) and human tonsil cells (right) with Anti-Human CD19 eFluor® 450 (Product # 48-0198-42) and Anti-Human CD307d (FcRL4) PerCP-eFluor® 710 (Product # 46-3079-42). Cells in the lymphocyte gate were used for analysis. Data courtesy of the laboratory of Shane Crotty.