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- Product number
- PA5-25293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYHIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma.
Wang S, Li F, Fan H
Scientific reports 2021 Oct 1;11(1):19593
Scientific reports 2021 Oct 1;11(1):19593
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PYHIN1 polyclonal antibody (Product # PA5-25293) in MDA-MB435 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MDA-MB435 cells using a PYHIN1 polyclonal antibody (Product # PA5-25293) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Characteristic IFIX expression in OSCC and its precursor stages. ( a - c ) IFIX expression by IHC in histopathologically assessed normal mucosa, hyperplasia, and dysplasia. ( d , e ) Lower expression of IFIX in moderately differentiated OSCC. ( f , g ) Higher expression of IFIX in well-differentiated OSCC cells. ( h ) The pattern of immunoreactive scores of IFIX staining in cancer tissue samples of OSCC at various histopathological grades and OSCC precursor stages.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Overexpression of IFIX induces OSCC apoptosis and suppresses cell invasion. ( a ) IFIX expression in CAL-27-NC and CAL-27-OE cells by western bloting and the blots were cut prior to hybridisation with antibodies during blotting. ( b ) Analysis of apoptosis induced in OSCC by overexpression of IFIX. Representative flow plots are shown. Apoptosis was analyzed using a FACS Calibur flow cytometer (BD Biosciences, San Jose, CA, USA) with a FITC Annexin V Apoptosis Detection kit (BD Biosciences). ( c ) Apoptosis rate measured by annexin V staining and determined by flow cytometry. The apoptosis percentage is presented as the mean +- SEM (n = 3); * P < 0.05 vs. control. All data were analyzed using FCS Express software (version 3.0; De Novo Software, Pasadena, CA, USA). ( d ) The wound healing assay indicates that overexpression of IFIX does not inhibit CAL-27 cell migration (magnification 100 x). Representative pictures are given. ( e ) Data are presented as the mean +- SD (n = 3); * P < 0.05 vs. control. ( f ) Transwell assay revealing that IFIX overexpression significantly inhibited CAL-27 cell invasion. Samples from three independent assays and representative pictures are shown (magnification 100 x or 200 x). ( g , h ) Graphs indicate invading cells in fields of view and an invasion fold change .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 IFIX does not limit the establishment of CSCs. ( a ) Cell lysates were analyzed by western blotting for CD133, NANOG, PIWIL2, and NOTCH1 as general CSC markers in CAL-27 cells infected with the lentivirus overexpressing IFIX (CAL-27-OE), CAL-27 cells infected with the empty lentiviral vector (CAL-27-vector), and CAL-27 normal control cancer cells (CAL-27-NC) Gels and blots were presented by three groups at a time and the blots were cut prior to hybridisation with antibodies during blotting. ( b - f ) The data were replicated twice in one test and presented as the mean +- SD; * P < 0.05.
