PA5-27076

antibody from Invitrogen Antibodies

Targeting: GPBAR1 BG37, GPCR, GPCR19, GPR131, M-BAR, MGC40597, TGR5

Antibody data

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Validation data

Product number: PA5-27076 - Provider product page
Provider: Invitrogen Antibodies
Product name: TGR5 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GPBAR1 protein structure - PA5-27076 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of TGR5 Antibody (Product # PA5-27076) was performed on a mouse (Mus musculus) adrenal gland. To expose target proteins, antigen retrieval was performed by microwaving tissues for 10 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide for 10 min at room temperature. Tissue slides were washed with deionized water and TBST, and then blocked in a protein free blocking buffer for 7 min at room temperature. Tissues were incubated with a TGR5 Antibody (Product # PA5-27076) diluted 1:100 in distilled water or rabbit IgG as a negative control for 1 hour at room temperature in a humidified chamber. Tissues were washed extensively in TBST and detection was performed using a polymer, and then followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were taken on a microscope at 20X magnification. In the image, immunolabeling is observed in the neurons of the adrenal gland medulla.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of TGR5 Antibody (Product # PA5-27076) was performed on a mouse adrenal gland. Antigen retrieval was performed by microwaving tissues for 10 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide for 10 min at room temperature. Tissue slides were washed with deionized water and TBST, and then blocked in a protein free blocking buffer for 7 min at room temperature. Tissues were incubated with a TGR5 Antibody (Product # PA5-27076) diluted 1:100 in distilled water or rabbit IgG as a negative control for 1 hour at room temperature in a humidified chamber. Tissues were washed extensively in TBST and detection was performed using a polymer, then followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were taken at 20X magnification. In the image, immunolabeling is observed in the neurons of the adrenal gland medulla. Data courtesy of Antibody Data Exchange Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded HUH-7 xenograft, using TGR5 (Product # PA5-27076) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.