MA5-17071

antibody from Invitrogen Antibodies

Targeting: EIF4B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17071

Provider

Invitrogen Antibodies

Product name

eIF4B Monoclonal Antibody (1F5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17071 targets EIF4B in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

Antibody clone number

1F5

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EIF4B using a EIF4B monoclonal antibody (Product # MA5-17071) against a human EIF4B (AA: 381-585) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EIF4B using EIF4B monoclonal antibody (Product # MA5-17071) in A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EIF4B using a EIF4B monoclonal antibody (Product # MA5-17071) against a human EIF4B (AA: 381-585) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EIF4B using EIF4B monoclonal antibody (Product # MA5-17071) in A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH3T3 cells using EIF4B monoclonal antibody (Product # MA5-17071) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B monoclonal antibody (Product # MA5-17071) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using EIF4B monoclonal antibody (Product # MA5-17071) (green) and negative control (red).